Enolase 3/ENO3 Protein, Human (His)

Enolase 3/ENO3 protein acts as a glycolytic enzyme, promoting the conversion of 2-phosphoglycerate into phosphoenolpyruvate. In addition to its role in glycolysis, ENO3 appears to play a crucial role in the development and regeneration of striated muscle. Enolase 3/ENO3 Protein, Human (His) is the recombinant human-derived Enolase 3/ENO3 protein, expressed by E. coli , with N-His labeled tag. The total length of Enolase 3/ENO3 Protein, Human (His) is 434 a.a., with molecular weight of ~45 kDa.

Enolase 3 (ENO3) is a glycolytic enzyme responsible for catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. Beyond its classical role in energy metabolism, ENO3 appears to play a specific function in striated muscle development and regeneration. The enzyme's involvement in these processes suggests a role in supporting the energetic demands of muscle tissues during development and repair. This dual functionality highlights the multifaceted roles of ENO3 in both fundamental metabolic pathways and specialized cellular processes related to muscle development and regeneration.

Specific activity is 5235 pmol/min/µg, and was obtained by measuring the decrease of NAD that incubate at 37 ºC for 5 minutes in absorbance at 340 nm resulting from NADH at pH 6.5.

Human

E. coli

N-10*His

P13929-1 (M1-K434)

2027  [NCBI]

MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

Approximately 50 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.