Fas/CD95 Protein, Human (Cell-Free, His)

The Fas/CD95 protein is a receptor for TNFSF6/FASLG and can initiate apoptotic events that are critical for programmed cell death. FADD promotes the recruitment of caspase-8 to form the death-inducing signaling complex (DISC) and activate caspases that mediate apoptosis. Fas/CD95 Protein, Human (Cell-Free, His) is the recombinant human-derived Fas/CD95 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Fas/CD95 Protein, Human (Cell-Free, His) is 310 a.a., with molecular weight of 40.6 kDa.

Fas, also known as CD95, serves as a receptor for TNFSF6/FASLG, initiating a cascade of apoptotic events crucial for programmed cell death. The adapter molecule FADD facilitates the recruitment of caspase-8 to the activated receptor, forming the death-inducing signaling complex (DISC) and initiating the cascade of caspases that mediate apoptosis. FAS-mediated apoptosis is implicated in peripheral tolerance induction and the antigen-stimulated suicide of mature T-cells. Additionally, secreted isoforms 2 to 6 of Fas have been identified, which function to block apoptosis in vitro. Fas engages in diverse protein interactions, including DAXX, HIPK3, RIPK1, FAIM2, BABAM2, FEM1B, FADD, NOL3, and CALM, forming a complex network that regulates cell death signaling. The interaction with various molecules highlights the multifaceted roles of Fas in cellular processes, emphasizing its significance in immune regulation and apoptosis modulation.

Human

E. coli Cell-free

N-10*His

P25445 (Q26-V335)

355

QVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV

40.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.