NEK9 Protein, Human (Sf9, GST)

The NEK9 protein is a pleiotropic mitotic regulator that oversees spindle dynamics and chromosome segregation. It phosphorylates a variety of substrates, including histones, myelin basic protein, β-casein, and BICD2. NEK9 Protein, Human (Sf9, GST) is the recombinant human-derived NEK9 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of NEK9 Protein, Human (Sf9, GST) is 978 a.a., .

NEK9, a pleiotropic regulator of mitotic progression, orchestrates critical processes in spindle dynamics and chromosome separation. Its pivotal role extends to phosphorylating various substrates, including histones, myelin basic protein, beta-casein, and BICD2. Specifically, NEK9 exhibits phosphorylation activity towards histone H3 on serine and threonine residues and beta-casein on serine residues. The multifaceted influence of NEK9 is underscored by its involvement in the G1/S transition and S phase progression. Moreover, NEK9 plays a key role in modulating the activity of NEK6 and NEK7, enhancing their functionality by releasing the autoinhibitory functions of Tyr-108 and Tyr-97, respectively. This comprehensive regulatory role emphasizes NEK9's significance in governing mitotic processes and ensuring the precise orchestration of cellular events during cell cycle progression.

Human

Sf9 insect cells

N-GST

Q8TD19 (S2-L979)

91754

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.