1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. PDGFs & PDGFRs
  4. PDGF
  5. PDGF-A
  6. PDGFAA Protein, Canine (P.pastoris, His)

PDGFAA Protein, Canine (P.pastoris, His)

Cat. No.: HY-P75965
COA Handling Instructions

PDGFAA Protein is platelet-derived growth factor subunit A precursor. PDGFAA Protein, Canine (P.pastoris, His) is the recombinant canine-derived PDGFAA protein, expressed by P. pastoris , with N-His labeled tag. The total length of PDGFAA Protein, Canine (P.pastoris, His) is 110 a.a., with molecular weight of ~14.5 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

PDGFAA Protein is platelet-derived growth factor subunit A precursor. PDGFAA Protein, Canine (P.pastoris, His) is the recombinant canine-derived PDGFAA protein, expressed by P. pastoris , with N-His labeled tag. The total length of PDGFAA Protein, Canine (P.pastoris, His) is 110 a.a., with molecular weight of ~14.5 kDa.

Background

PDGFAA Protein is platelet-derived growth factor subunit A precursor. PDGFAA enables growth factor activity and platelet-derived growth factor receptor binding. PDGFAA is involved in platelet-derived growth factor receptor signaling pathway, positive regulation of MAP kinase activity and positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction[1][2][3].

Species

Canine

Source

P. pastoris

Tag

N-His

Accession

NP_001177101 (S87-R196)

Gene ID

491597  [NCBI]

Molecular Construction
N-term
His
PDGFAA (S87-R196)
Accession # NP_001177101
C-term
Synonyms
Platelet-derived growth factor subunit A; PDGF-1; PDGF-A; Rpa1
Molecular Weight

Approximately 14.5 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 30% CAN, 0.1% TFA. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PDGFAA Protein, Canine (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PDGFAA Protein, Canine (P.pastoris, His)
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HY-P75965
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