ZP3 Protein, Human (HEK293, His)

ZP3 protein, a vital component of the zona pellucida, is crucial for sperm binding, inducing the acrosome reaction, and preventing polyspermy. Alongside ZP1, ZP2, and ZP4, it forms a glycoprotein complex in the zona matrix, organizing into filaments cross-linked by ZP1 homodimers. ZP3 actively interacts with ZP1 and ZP2, highlighting its integral role in the molecular architecture governing fertilization processes. ZP3 Protein, Human (HEK293, His) is the recombinant human-derived ZP3 protein, expressed by HEK293 , with N-His, N-Myc labeled tag. The total length of ZP3 Protein, Human (HEK293, His) is 365 a.a., with molecular weight of approximately 55-75 kDa.

As a crucial element of the zona pellucida, ZP3 protein contributes to the extracellular matrix surrounding oocytes, playing a pivotal role in mediating sperm binding, inducing the acrosome reaction, and preventing post-fertilization polyspermy. Within the zona pellucida, ZP3, along with ZP1, ZP2, and ZP4, forms a glycoprotein complex essential for sperm binding and the formation of the zona matrix. ZP2 and ZP3 polymers organize into long filaments cross-linked by ZP1 homodimers, highlighting the structural intricacies of the zona pellucida matrix. Moreover, ZP3 actively interacts with ZP1 and ZP2, underscoring its integral role in the complex molecular architecture that governs fertilization processes.

Human

HEK293

N-His;N-Myc

P21754 (23Q-387V)

7784  [NCBI]

QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSV

approximately 55-75 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 20% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.