Colorimetric Substrate

By Targets:	Biochemical Assay Reagents (1) Caspase (4) Elastase (1) Epoxide Hydrolase (1) Fluorescent Dye (2) Isotope-Labeled Compounds (1) Poly(ADP-ribose) Glycohydrolase (PARG) (2)
By Research Areas:	Cancer (1)

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-15926

ONPG 4 Publications Verification 2-Nitrophenyl β-D-galactopyranoside	Biochemical Assay Reagents	Others
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.

HY-P1006

Ac-DEVD-pNA

Caspase Others

Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases.

Ac-DEVD-pNA	Caspase	Others
Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases.

HY-126785

Suc-AAP-Abu-pNA Colorimetric Elastase Substrate	Elastase	Others
Suc-AAP-Abu-pNA (Colorimetric Elastase Substrate) is a specific substrate for pancreatic elastase (K_m = 100 μM; K_cat/K_m = 35,300 s ^-1 M ^-1 for rat pancreatic elastase; K_m = 30 μM; K_cat/K_m = 351,000 s ^-1 M ^-1 for porcine pancreatic elastase). Suc-AAP-Abu-pNA also promotes OPC migration .

HY-121549

S-NEPC 4-Nitrophenyl-2S,3S-epoxy-3 phenylpropyl carbonate	Epoxide Hydrolase	Others
S-NEPC (4-Nitrophenyl-2S,3S-epoxy-3 phenylpropyl carbonate) is a colorimetric substrate used to measure epoxide hydrolase activity .

HY-134354

pNP-ADPr ADP-ribose-pNP	Poly(ADP-ribose) Glycohydrolase (PARG)	Others
pNP-ADPr is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays. pNP-ADPr can be used for the research of poly(ADP-ribose)polymerase (PARP) enzymes .

HY-P10236

Ac-WEHD-pNA

Caspase Others

Ac-WEHD-pNA is a colorimetric substrate for caspase 1, 4, 5 and 14, which is utilized to measure the caspase activity .

Ac-WEHD-pNA	Caspase	Others
Ac-WEHD-pNA is a colorimetric substrate for caspase 1, 4, 5 and 14, which is utilized to measure the caspase activity .

HY-15909

BCIP BCIP p-toluidine salt; X-phosphate p-toluidine salt	Fluorescent Dye	Others
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

HY-15926S

ONPG-13C 2-Nitrophenyl β-D-galactopyranoside-¹³C	Isotope-Labeled Compounds	Others
ONPG- ¹³C is the ¹³C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit[1][2].

HY-P10001

Z-IETD-pNA Z-Ile-Glu-Thr-Asp-pNA	Caspase	Cancer
Z-IETD-pNA (Z-Ile-Glu-Thr-Asp-pNA) is a colorimetric caspase-8 and granzyme B substrate. Z-IETD-pNA is hydrlyzed by caspase 8 to generate pNA .

HY-D1224

Chlorophenol red-β-D-galactopyranoside	Fluorescent Dye	Others
Chlorophenol red-β-D-galactopyranoside is a long-wavelength dye, which has been widely used for colorimetric assays. Chlorophenol red-β-D-galactopyranoside is also a β-galactosidase substrate commonly used in recombinant yeast oestrogenic assays .

HY-120833

Ac-Ile-Glu-Thr-Asp-pNA	Caspase	Others
Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .

HY-134354A

pNP-ADPr disodium ADP-ribose-pNP disodium	Poly(ADP-ribose) Glycohydrolase (PARG)	Others
pNP-ADPr disodium is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays. pNP-ADPr disodium can be used for the research of poly(ADP-ribose)polymerase (PARP) enzymes .

Cat. No.	Product Name	Type

HY-15909

BCIP BCIP p-toluidine salt; X-phosphate p-toluidine salt	Chromogenic Substrates
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.

HY-D1224

Chlorophenol red-β-D-galactopyranoside	Dyes
Chlorophenol red-β-D-galactopyranoside is a long-wavelength dye, which has been widely used for colorimetric assays. Chlorophenol red-β-D-galactopyranoside is also a β-galactosidase substrate commonly used in recombinant yeast oestrogenic assays .

Cat. No.	Product Name	Type

HY-15926

ONPG 4 Publications Verification 2-Nitrophenyl β-D-galactopyranoside	Enzyme Substrates
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.

Cat. No.	Product Name	Target	Research Area

HY-126785

Suc-AAP-Abu-pNA Colorimetric Elastase Substrate	Elastase	Others
Suc-AAP-Abu-pNA (Colorimetric Elastase Substrate) is a specific substrate for pancreatic elastase (K_m = 100 μM; K_cat/K_m = 35,300 s ^-1 M ^-1 for rat pancreatic elastase; K_m = 30 μM; K_cat/K_m = 351,000 s ^-1 M ^-1 for porcine pancreatic elastase). Suc-AAP-Abu-pNA also promotes OPC migration .

HY-P10143

MMP-2/MMP-9 Substrate

Peptides Others

MMP-2/MMP-9 Substrate, a synthetic colorimetric substrate, is a peptide .
HY-P5992

Factor VIIa substrate

Peptides Others

Factor VIIa substrate is a colorimetric substrate for Factor VIIa, that can be measured by absorbance at 405 nm .
HY-P10059

Boc-Val-Gly-Arg-βNA

Peptides Others

Boc-Val-Gly-Arg-βNA is a colorimetric substrate for plasminogen activator .

MMP-2/MMP-9 Substrate	Peptides	Others
MMP-2/MMP-9 Substrate, a synthetic colorimetric substrate, is a peptide .

Factor VIIa substrate	Peptides	Others
Factor VIIa substrate is a colorimetric substrate for Factor VIIa, that can be measured by absorbance at 405 nm .

Boc-Val-Gly-Arg-βNA	Peptides	Others
Boc-Val-Gly-Arg-βNA is a colorimetric substrate for plasminogen activator .

HY-P5348

Ac-LEHD-pNA	Peptides	Others
Ac-LEHD-pNA is a biological active peptide. (Caspase-9 substrate; pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm.)

HY-P1006

Ac-DEVD-pNA

Caspase Others

Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases.
HY-P4335

Bz-RGFFP-4MβNA

Peptides Others

Bz-RGFFP-4MβNA, a substrate for Cathepsin D, is suitable for either colorimetric or fluorometric assay .
HY-P10236

Ac-WEHD-pNA

Caspase Others

Ac-WEHD-pNA is a colorimetric substrate for caspase 1, 4, 5 and 14, which is utilized to measure the caspase activity .

Ac-DEVD-pNA	Caspase	Others
Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases.

Bz-RGFFP-4MβNA	Peptides	Others
Bz-RGFFP-4MβNA, a substrate for Cathepsin D, is suitable for either colorimetric or fluorometric assay .

Ac-WEHD-pNA	Caspase	Others
Ac-WEHD-pNA is a colorimetric substrate for caspase 1, 4, 5 and 14, which is utilized to measure the caspase activity .

HY-P10001

Z-IETD-pNA Z-Ile-Glu-Thr-Asp-pNA	Caspase	Cancer
Z-IETD-pNA (Z-Ile-Glu-Thr-Asp-pNA) is a colorimetric caspase-8 and granzyme B substrate. Z-IETD-pNA is hydrlyzed by caspase 8 to generate pNA .

HY-120833

Ac-Ile-Glu-Thr-Asp-pNA	Caspase	Others
Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .

Cat. No.	Product Name	Chemical Structure

HY-15926S

ONPG-13C

ONPG- ¹³C is the ¹³C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit[1][2].

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社
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Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Isotope-Labeled Compounds