2. Cell Cycle/DNA Damage MAPK/ERK Pathway Anti-infection Apoptosis
  3. DNA/RNA Synthesis JNK Bacterial Apoptosis Antibiotic Parasite
  4. Anisomycin

Anisomycin  (Synonyms: アニソマイシン; Flagecidin; Wuningmeisu C)

製品番号: HY-18982 純度: 99.82%
COA 取扱説明書 Technical Support

Anisomycin is a potent protein synthesis inhibitor which interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system. Anisomycin is a JNK activator, which increases phospho-JNK. Anisomycin is a bacterial antibiotic.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 22862-76-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 46 在庫あり
Solution
10 mM * 1 mL in DMSO USD 46 在庫あり
Solid
5 mg $42 在庫あり
10 mg $66 在庫あり
25 mg $99 在庫あり
50 mg $132 在庫あり
100 mg $198 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 138 publication(s) in Google Scholar

Other Forms of Anisomycin:

Anisomycin 関連抗体

Top Publications Citing Use of Products

顧客検証

WB

    Anisomycin purchased from MedChemExpress. Usage Cited in: Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C225-C235.  [Abstract]

    Western blot analysis shows that inhibiting MAPK cannot completely reverse increased expression of RNF2 and CDDP resistance of OC cells.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2018 Sep 5:834:295-304.  [Abstract]

    The protein levels of p-JNK by western blot following Anisomycin (4 μM) treatment for 24 h after Morroniside (100 μM) 12 h.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2018 Apr 23;8(1):6379.  [Abstract]

    Immunoblot analysis of lysates from Anisomycin pre-treatment cells reveals an increase of CHIP protein compared with OGD treated only cells, contrasting with a decrease in RIPK3 and p-MLKL protei. Pre-treatment cells with SP600125 results in the expected attenuation in JNK phosphorylation levels.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 May 23;499(4):743-750.  [Abstract]

    Astrocytes were cultured with p38 and JNK activator of DHC and ANS, respectively, at the indicated concentrations for 24 h. Then, all cells were harvested for western blot analysis of p-p38 and p-JNK. Astrocytes were pre-treated with DHC (500 nM) or ANS (10 μM) for 24 h, followed by Fru (5 mM) for an additional 24 h. Then, the following experiments were performed.

    Anisomycin purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):467-473.  [Abstract]

    The increase of Bcl-2 and decrease of Bax, cleaved Caspase-3 and PARP induced by Ulk1- inhibition in LPS-exposed AST are abrogated by the Dehydrocorydaline chloride (DHC) or Anisomycin (ANS) pre-treatment

    Anisomycin purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2017 Nov 1:277:62-73.  [Abstract]

    Effects of silencing p53 on colistin-induced ROS production and p-JNK expression level in PC-12 cells. The protein levels of p53. The protein levels of p-JNK by western blot.

    DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

    JNK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    JNK1 JNK2 JNK3 JNK

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    Parasite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Anisomycin is a potent protein synthesis inhibitor which interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system[1]. Anisomycin is a JNK activator, which increases phospho-JNK[2][3]. Anisomycin is a bacterial antibiotic[4].

    IC50 & Target[1][3]

    JNK

     

    DNA synthesis

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 IC50
    0.02 μM
    Compound: 1
    Inhibitory concentration required to produce cytotoxicity against HEK293 cells
    Inhibitory concentration required to produce cytotoxicity against HEK293 cells
    		[PMID: 16005213]
    Huh-7 CC50
    >2.357 μM
    Compound: GNF-Pf-4549
    NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
    NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
    		[PMID: 18579783]
    Vero C1008 CC50
    <0.39 μM
    Compound: Anisomycin
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 CC50
    <0.39 μM
    Compound: Anisomycin
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    体外実験

    To examine whether JNK has a core role in colistin-induced neurotoxicity in PC-12 cells, an SP600125 (a highly selective inhibitor of JNK) and Anisomycin (a potent activator) are used in this study. In order to select an appropriate concentration, PC-12 cells are treated with a range of SP600125 (0-80 μM) and Anisomycin (0-20 μM) respectively for 24 h. The results show that the cells viability significantly decreases by SP600125 treatment in a concentration-dependent manner, observed at the concentrations greater than 20 μM (p<0.01). Similarly the cells viability is inhibited by Anisomycin treatment (≥8 μM) (p<0.05) [1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Anisomycin 関連抗体
    体内実験

    Anisomycin (60 mg/kg; for 4-week continuous intravenous administration) significantly decreases mouse body weight in a dose-related manner, compared with the control group. Anisomycin (15 mg/kg; for 4-week continuous intravenous administration) slightly and transiently decreases the mouse body weight. There is no significant difference of the mouse body weight in 5 mg/kg group[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Balb/c mice of both sexes (4-5 weeks old)[3]
    Dosage: 84, 99, 116, 136 or 160 mg/kg; 0.2 mL per mouse
    Administration: Intravenously injected through mouse tail vein
    Result: The calculated LD50 for Anisomycin was 119.64 mg/kg.
    分子量

    265.31

    分子式

    C14H19NO4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@@H](OC(C)=O)[C@@H](CC2=CC=C(OC)C=C2)NC1
    Structure Classification
    Initial Source

    Streptomyces griseolus
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (188.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 4 mg/mL (15.08 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7692 mL 18.8459 mL 37.6918 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL
    10 mM 0.3769 mL 1.8846 mL 3.7692 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 4 mg/mL (15.08 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.82%

    COA (252 KB) HNMR (247 KB) RP-HPLC (243 KB) LCMS (86 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.7692 mL 18.8459 mL 37.6918 mL 94.2294 mL
    5 mM 0.7538 mL 3.7692 mL 7.5384 mL 18.8459 mL
    10 mM 0.3769 mL 1.8846 mL 3.7692 mL 9.4229 mL
    15 mM 0.2513 mL 1.2564 mL 2.5128 mL 6.2820 mL
    DMSO 20 mM 0.1885 mL 0.9423 mL 1.8846 mL 4.7115 mL
    25 mM 0.1508 mL 0.7538 mL 1.5077 mL 3.7692 mL
    30 mM 0.1256 mL 0.6282 mL 1.2564 mL 3.1410 mL
    40 mM 0.0942 mL 0.4711 mL 0.9423 mL 2.3557 mL
    50 mM 0.0754 mL 0.3769 mL 0.7538 mL 1.8846 mL
    60 mM 0.0628 mL 0.3141 mL 0.6282 mL 1.5705 mL
    80 mM 0.0471 mL 0.2356 mL 0.4711 mL 1.1779 mL
    100 mM 0.0377 mL 0.1885 mL 0.3769 mL 0.9423 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Anisomycin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Anisomycin22862-76-6Flagecidin Wuningmeisu CDNA/RNA SynthesisJNKBacterialApoptosisAntibioticParasitec-Jun N-terminal kinaseInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Anisomycin
    製品番号:
    HY-18982
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.