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Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.

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Safflower yellow Chemical Structure

Safflower yellow Chemical Structure

CAS No. : 1401-20-3

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Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Safflower yellow

WB

    Safflower yellow purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Nov;107:1736-1743.  [Abstract]

    Western blot is used to assess the protein levels of VEGF, Ang-2, ALP, Runx2, and OPN-1 in the co-culture of HUVEC-12 and BMSCs. β-actin is used as a loading control.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.

    In Vivo

    Safflower yellow (SY) is the safflower extract and is the one of traditional Chinese medicine. Safflower yellow can promote blood circulation, remove blood stasis, and thereby improve capillary circulation at the site of tissue injury. Safflower yellow is mixtures of a water-soluble chalcone component, in which both hydroxyl safflower yellow A (HSYA) and safflower yellow B (SYB) are the main components. Safflower injection excellently protects the heart by way of improving functions of cardiac contraction and dilation, increasing coronary blood flow, and strengthening the bcl-2 (anti apoptosis gene) protein expression. Safflower yellow alleviates the injured tendon adhesion and inflammatory reaction and promoted the repair of injured tendon[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CAS No.
    Appearance

    Solid

    Color

    Light brown to brown

    Emission (Em)

    500

    Excitation (Ex)

    320

    SMILES

    [Safflower yellow]

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 20 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References
    Animal Administration
    [1]

    Rabbits[1]
    The adult male New Zealand rabbits (body weight 2.0-2.5 kg) are used. Twenty-four rabbits are randomly divided into three groups (per group): sham-operated control (Cont), spinal cord ischemia reperfusion, and treated with safflower yellow. The control group only execute anesthesia and surgical procedures, except for occluding the abdominal aorta. The group is intravenously injected with 2 mL/kg of a solution of 16% (wt/vol) Safflower yellow (1 mL, containing 1.6 mg Safflower yellow), followed by continuous infusion of a total of 5 mL/kg through the right femoral vein at the moment of reperfusion beginning after 40 minutes of the abdominal aorta occlusion. The same volumes of 0.9% saline solution are administrated in control and groups. Blood samples are obtained at the end of 0 hour, 4 hours, 12 hours, 24 hours, and 48 hours after reperfusion, and the plasma is separated and stored at -80°C for further analysis. All animals are sacrificed 48 hours after reperfusion and are rapidly perfused with 0.9% sodium chloride, and the L2-5 segments of the spinal cord are quickly removed. The L2-3 segment in each animal is used for western blot, and the other segment (L4-5) is immersed into 10% neutral formaldehyde for 2-3 days and is used for morphology analysis[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Safflower yellow Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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