2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Proliferation and Cytotoxicity
  6. Cell-ATP Viability Detection Kit

Cell-ATP Viability Detection Kit 

Cat. No.: HY-K0302
Manual COA SDS Technical Support
Culture Assay

MCE Cell-ATP Viability Detection Kit is used for detecting the number and viability of living cells in culture based on high-sensitivity bioluminescence detection technology of the ATP present.Product specifications: Large sizes are based on 100 mL increments.

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    Cell-ATP Viability Detection Kit purchased from MCE. Usage Cited in: J Nanobiotechnology. 2025 Dec 6;23(1):767.  [Abstract]

    Quantification of intracellular ATP levels.

    Cell-ATP Viability Detection Kit purchased from MCE. Usage Cited in: Pharmacol Res. 2025 Jan 21:212:107620.  [Abstract]

    Pre-treatment of 266–6 cells with Emo (2.5–10 μM) for 12 h and then exposed to NaT (5 mM) for 3 h; Cell viability was determined using the Cell-ATP Viability Detection Kit.

    Cell-ATP Viability Detection Kit purchased from MCE. Usage Cited in: J Ethnopharmacol. 2025 Sep 3:120526.  [Abstract]

    Assessment of liquiritigenin toxicity in 266–6 cells using ATP viability .

    Cell-ATP Viability Detection Kit purchased from MCE. Usage Cited in: bioRxiv. 2025 Nov 18.

    A375 cells transduced with empty vector control (Ctrl) or shRNA targeting both GNAQ and GNA11 (shGαq/11) were treated with vehicle or trametinib for 72 h. Cell viability was measured by CellTiter-Glo, and dose-response curves are shown.

    • Description

    • Storage

    • Application

    • Components

    • Documentation

    Description
    & Advantages

    ATP is an important index of cell metabolism which has a good linear relationship with the number of living cells. The principle of ATP bioluminescence technology is as follows: luciferase uses luciferin, adenosine triphosphate (ATP) and O2 as substrates, and converts chemical energy into light energy in the presence of Mg2+. In the luminescence reaction catalyzed by luciflucase, the concentration of ATP is linearly related to luminescence intensity within a certain concentration range. The amount of ATP is directly proportional to the number of cells present. Based on this, the Cell-ATP Viability Detection Kit can be used for cell counting or viability determination by ATP content.

    MCE Cell-ATP Viability Detection Kit is used for detecting the number and viability of living cells in culture based on high-sensitivity bioluminescence detection technology of the ATP present.

    Storage

    -20°C, one year.

    Keep away from light.

    Shipping with dry ice
    Application
    Cell viability assay; Live cell counting
    Components
    Components HY-K0302-10 mL HY-K0302-100 mL HY-K0302-500 mL
    Cell-ATP Viability Detection Reagent 10 mL 100 mL 100 mL*5
    Documentation

    Manual (137 KB) SDS (214 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CTGATP Luminescence Sensitive Cell Viability Assay KitCell ATP Content AssayATP DetectionLuminescent ATP Detection Kit

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