1. Cell Cycle/DNA Damage
    NF-κB
  2. PPAR

L-165041 

Cat. No.: HY-20019 Purity: 99.14%
Data Sheet SDS Handling Instructions

L-165041 is a cell permeable PPARδ agonist which induces adipocyte differentiation in NIH-PPARδ cells.

For research use only. We do not sell to patients.
L-165041 Chemical Structure

L-165041 Chemical Structure

CAS No. : 79558-09-1

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $72 In-stock
5 mg $65 In-stock
10 mg $95 In-stock
50 mg $380 In-stock
100 mg $630 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Description

L-165041 is a cell permeable PPARδ agonist which induces adipocyte differentiation in NIH-PPARδ cells.

In Vitro

L-165041 (1 or 5 µM) inhibits VEGF-induced EC proliferation and migration. L-165041 negatively affects cell cycle progression in VEGF-activated HUVECs. L-165041 inhibits PPARδ-independent, VEGF-induced angiogenesis[1]. PPARδ ligand L-165041 inhibits PDGF-induced rVSMC proliferation and migration. With 1 h of L-165041 pretreatment, PDGF-induced cellular migration is inhibited. L-165041 (10 μM) significantly suppresses S phase transition induced by PDGF[3].

In Vivo

L-165041 (10 µM) inhibits VEGF-induced angiogenesis ex vivo and in vivo. Mice aortic rings treated with L-165041 show significantly attenuated EC outgrowth compared to control aortic rings[1]. In L-165041- (5 mg/kg/day, i.p.) treated mice, the formation of lipid droplets is significantly lower. L-165041 treatment significantly reduces the level of both the hepatic cholesterol and triglycerides in mice. L-165041 increases mRNA expression levels of PPARδ compared to the vehicle group. Lipoprotein lipase (LPL) expression in L-165041-treated mice is significantly higher than that in the vehicle group[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.4848 mL 12.4242 mL 24.8484 mL
5 mM 0.4970 mL 2.4848 mL 4.9697 mL
10 mM 0.2485 mL 1.2424 mL 2.4848 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

For Western blot analysis, VEGF-stimulated cells with or without L-165041 pretreatment are washed with phosphate-buffered saline (PBS) and scraped in 1× protein lysis buffer. Protein extracts from each group are separated in an SDS-PAGE gel and then transferred onto PVDF membranes. The blots are incubated with appropriate primary antibodies. β-Actin is used to correct for loading errors. Immunopositive bands are visualized by ECL. The relative protein amount is determined by densitometric analysis. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

L-165041 is formulated in 0.1 N NaOH.

LDLR−/− mice are divided into vehicle (0.1 N NaOH) and L-165041 (5 mg/kg/day) group (9 animals in each group). LDLR−/− mice receive either NaOH or L-165041 via daily intraperitoneal injection (i.p.) for 16 weeks with the Western diet. Body weight is measured once a week and the blood samples for a serum parameter analysis are collected using an eye-bleeding method every 4 weeks. At the end of the experiment, LDLR−/− mice are fasted for 24 h before sacrificed and the liver samples are either fixed in formalin or frozen at −70°C for further analysis. All animals are housed in polycarbonate cages in a room with a 12-h light/12-h dark cycle, and maintained at a constant temperature of 22°C. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

402.44

Formula

C₂₂H₂₆O₇

CAS No.

79558-09-1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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L-165041
Cat. No.:
HY-20019
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