VHL Antibody (YA5693)

Cat. No.: HY-P86001: Data Sheet COA
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VHL Antibody (YA5693) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to VHL.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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1 Publications Citing Use of MCE VHL Antibody (YA5693)

•J Pharm Anal. 2025 Dec 11.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

VHL Antibody (YA5693) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to VHL.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 24 kDa,19 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P40337

Gene ID

7428 [NCBI]

Immunogen

Synthesized peptide derived from human pVHL AA range: 150-213

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:500-5000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of MCF-7cells labeling VHL Antibody with VHL Antibody (YA5693) (HY-P86001) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with VHL Antibody (YA5693) ((HY-P86001) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF-7 cells labeling VHL Antibody with VHL Antibody (YA5693) (HY-P86001) at 1/100dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with VHL Antibody (YA5693) ((HY-P86001) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed:10944113, PubMed:17981124, PubMed:19584355). Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions (PubMed:10944113, PubMed:17981124). Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases (PubMed:10944113, PubMed:17981124). Ubiquitinates, in an oxygen-responsive manner, ADRB2 (PubMed:19584355). Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR (PubMed:34290272)

Subcellular Localization：Cytoplasm; Cell membrane; Peripheral membrane protein; Endoplasmic reticulum; Nucleus; Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is expressed in both the human brain and kidneys.

Isoforms & Post-Translational Modification：P40337 has 3 isomers: P40337-1: 24153 Da (predicted); P40337-2: 19654 Da (predicted); P40337-3: 18532 Da (predicted).

Subunit：Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome-dependent degradation of targeted proteins.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)