LDN193189 Tetrahydrochloride (Synonyms: DM-3189 Tetrahydrochloride)

Name: LDN193189 Tetrahydrochloride
Brand: MedChemExpress
SKU: HY-12071A
Rating: 5.0 (83 reviews)

Cat. No.: HY-12071A Purity: 99.79%: Data Sheet
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LDN193189 (DM-3189) Tetrahydrochloride is a potent selective BMP type I receptor (BMP I) inhibitor. LDN193189 efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC₅₀ values of 5 nM and 30 nM, respectively. LDN193189 can be used for the research of bone morphogenetic protein signalling, such as fibrodysplasia ossificans progressiva.

For research use only. We do not sell to patients.

CAS No. : 2310134-98-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 83 publication(s) in Google Scholar

Other Forms of LDN193189 Tetrahydrochloride:

LDN193189 In-stock
LDN193189 dihydrochloride In-stock
LDN193189 Tetrahydrochloride (Standard) Get quote

83 Publications Citing Use of MCE LDN193189 Tetrahydrochloride

2D/3D Cell Culture and Differentiation

Cell Migration/Invasion Assay

IHC

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]; The bar chart displayed the weighted average of day 13 budoid proportions exhibiting no elongation, elongation (short), or elongation (long) after treatment with LDN193189 Tetrahydrochloride (1-4 μM).

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 28;11(48):eady7682. [Abstract]; Max-projection confocal images of day 13 budoids were treated with LDN193189 Tetrahydrochloride (LDN) (1 μM). Gray: Hoechst; magenta: SOX9.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]; Phospho-Smad1/5/9, COL1A1 and RUNX2 were quantified in hBM-MSCs after 3 days of treatment with LDN193189 (100 nM; 3 days) by western blot analysis and normalized to GAPDH and tSmad1.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]; LDN193189 (100 nM; 3 days). Representative images and quantification of immunofluorescence staining of COL1A1. Scale bars, 100 μm.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424. [Abstract]; LDN193189 (100 nM; 3 days). Representative images of ARS.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]; LDN193189 Tetrahydrochloride (0.2–0.3 μM; 24 h) blocked Smad1/5/9 phosphorylation in KYSE30 and KYSE150 cells.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]; LDN193189 Tetrahydrochloride (LDN) (0.2–0.3 μM; 24 h) inhibited the migration of KYSE30 and KYSE150 cells.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2020 Jan 28;469:390-398. [Abstract]; Transwell assays were performed on SERPINE2-overexpressing KYSE30 and KYSE150 cells treated with or without LDN193189 Tetrahydrochloride (0.2–0.3 μM; 24 h) (LDN) in the lower chamber.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2020 Jun 8;2020:4175613. [Abstract]; Western blot and grey analysis of CYP11a1, CYP19a1, BCL2, CALR, CHOP, caspase3, caspase12, PERK, and GRP78. qRT-PCR analyzed ATF4, caspase3, and GRP78 mRNAs. The relieving function of BMP4 on ER stress is diminished after blockade of BMP receptor I (BMPRI) with DM-3189.

: LDN193189 Tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cardiovasc Toxicol. 2019 Jun;19(3):264-275. [Abstract]; H9c2 cells are treated as following: control, DOX, DOX + rhBMP (Bmpr1a agonist) and DOX + LDN193189 (Bmpr1a inhibitor). Cells with red fluorescence indicate favorable MMP while green fluorescence indicates damage.

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ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B BMP

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

ACVR1

5 nM (IC₅₀)

BMPR1A

30 nM (IC₅₀)

In Vitro

LDN193189 Tetrahydrochloride efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC₅₀ values of 5 nM and 30 nM, respectively^[1].
LDN193189 Tetrahydrochloride has weake effects on activin and the TGF-β type I receptors ALK4, ALK5 and ALK7 with IC₅₀ values of ≥ 500 nM^[1].
LDN193189 Tetrahydrochloride binds ActRIIA with K_d value of 14 nM^[2].
LDN193189 Tetrahydrochloride (0.5 μM; 30 min) targets GDF8 induced Smad2/3 signaling and repression of myogenic transcription factors^[2].
LDN193189 Tetrahydrochloride (0.05, 0.5, 5 μM) efficiently inhibits GDF8 induced Smad3/4 reporter gene activity^[2].
LDN193189 Tetrahydrochloride (0-5 μM) rescues myogenesis in myoblasts treated with GDF8^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

LDN193189 (i.p.; 3 mg/kg; daily; for 35 days) Tetrahydrochloride might affect the interaction between breast cancer cells and the bone environment^[3].
LDN193189 (i.p.; 3 mg/kg; single) Tetrahydrochloride shows a reduction in ectopic ossification and functional impairment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

552.33

Formula

C₂₅H₂₆Cl₄N₆

CAS No.

2310134-98-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

C1(C2=C3N=CC(C4=CC=C(N5CCNCC5)C=C4)=CN3N=C2)=CC=NC6=CC=CC=C16.Cl.Cl.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (60.34 mM; Need ultrasonic)

DMSO : 4.17 mg/mL (7.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8105 mL	9.0526 mL	18.1051 mL
5 mM	0.3621 mL	1.8105 mL	3.6210 mL
10 mM	0.1811 mL	0.9053 mL	1.8105 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (286 KB) SDS (393 KB)

COA (258 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. Yu PB, et al. BMP type I receptor inhibition reduces heterotopic [corrected] ossification. Nat Med, 2008, 14(12), 1363-1369. [Content Brief]

[2]. Daniel Horbelt, et al. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem. 2015 Feb 6;290(6):3390-404. [Content Brief]

[3]. Julien Vollaire, et al. The Bone Morphogenetic Protein Signaling Inhibitor LDN-193189 Enhances Metastasis Development in Mice. Front Pharmacol. 2019 Jun 19;10:667. [Content Brief]

Cell Assay ^[1]	Mouse PASMCs grown are transiently transfected to 50% confluence in six-well plates with 0.3 μg Id1promoter luciferase reporter construct (BRE-Luc) in combination with 0.6 μg of plasmid expressing constitutively active forms of BMP type I receptors (caALK2, caALK3 or caALK6). For both reporter plasmids, 0.2 μg of pRL-TKRenilla luciferase are used to control for transfection efficiency. PASMCs are incubated with LDN193189 (2 nM-32 μM) or vehicle starting 1 h after transfection. Cell extracts are harvested and quantified relative promoter activity by the ratio of firefly to Renilla luciferase activity with the dual luciferase assay kit^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]^[3]	Mice^[2] In the first experiment, SCID mice are implanted with MDA-PCa-118b tumors. After 7 days when tumors reached measurable sizes, mice are injected with LDN193189 (3 mg/kg) or with vehicle intraperitoneally twice a day. Tumor sizes and body weights are measured weekly. Mice are injected with calcein at three days and one day prior to sacrifice. Blood is collected and tumors are weighed. A portion of the tumors are fixed in formaldehyde for micro-computed tomography (microCT), using EVS CT, or further decalcified for bone histomorphometric analysis, using OsteoMeasure Analysis System, or flash frozen for RNA preparation. Osteocalcin in the mouse serum is determined by ELISA. In the second experiment, PCa-118b tumors are first digested with Accumax, and the isolated cells are plated overnight, resuspended in Matrigel in 1:1 ratio, and injected into SCID mice (1×10⁶ cells/mouse) subcutaneously. Mice are treated with LDN193189 five days post-injection. Rats^[3] Male Sprague-Dawley (SD) rats, 8 weeks of age, weighing 200-220 g, are purchased from Nanjing Medical University animal center. Rats are randomly assigned to one of seven experiment groups (n=6 per group). Rats are housed with free access to food and water under a natural 12/12 h day/night cycle. The Monocrotaline is administered (60 mg/kg) to rats by subcutaneous injection into the back region. The animal’s lungs are harvested at 28th day of the study after hemodynamic assessment. The UK-92480 group received daily intragastric administration of UK-92480 after the administration of MCT (60 mg/kg). The LDN193189 group received daily intragastric administration of UK-92480 (50 mg/kg) and intraperitoneal injection of LDN193189 (10 mg/kg). In other groups, the same volume saline is given. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Yu PB, et al. BMP type I receptor inhibition reduces heterotopic [corrected] ossification. Nat Med, 2008, 14(12), 1363-1369. [Content Brief] [2]. Daniel Horbelt, et al. Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem. 2015 Feb 6;290(6):3390-404. [Content Brief] [3]. Julien Vollaire, et al. The Bone Morphogenetic Protein Signaling Inhibitor LDN-193189 Enhances Metastasis Development in Mice. Front Pharmacol. 2019 Jun 19;10:667. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.8105 mL	9.0526 mL	18.1051 mL	45.2628 mL
DMSO / H₂O	5 mM	0.3621 mL	1.8105 mL	3.6210 mL	9.0526 mL
H₂O	10 mM	0.1811 mL	0.9053 mL	1.8105 mL	4.5263 mL
	15 mM	0.1207 mL	0.6035 mL	1.2070 mL	3.0175 mL
	20 mM	0.0905 mL	0.4526 mL	0.9053 mL	2.2631 mL
	25 mM	0.0724 mL	0.3621 mL	0.7242 mL	1.8105 mL
	30 mM	0.0604 mL	0.3018 mL	0.6035 mL	1.5088 mL
	40 mM	0.0453 mL	0.2263 mL	0.4526 mL	1.1316 mL
	50 mM	0.0362 mL	0.1811 mL	0.3621 mL	0.9053 mL
	60 mM	0.0302 mL	0.1509 mL	0.3018 mL	0.7544 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

LDN193189 Tetrahydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LDN193189 Tetrahydrochloride2310134-98-4DM-3189 TetrahydrochlorideOrganoidTGF-β ReceptorTransforming growth factor beta receptorsInhibitorinhibitorinhibit

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LDN193189 Tetrahydrochloride (Synonyms: DM-3189 Tetrahydrochloride)

Customer Review

Other Forms of LDN193189 Tetrahydrochloride:

LDN193189 Tetrahydrochloride Related Antibodies

83 Publications Citing Use of MCE LDN193189 Tetrahydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All TGF-β Receptor Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

LDN193189 Tetrahydrochloride Related Antibodies

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Complete Stock Solution Preparation Table

LDN193189 Tetrahydrochloride Related Classifications

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