2. Academic Validation
  3. An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids

An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids

  • Curr Protoc Cell Biol. 2018 Dec;81(1):e62. doi: 10.1002/cpcb.62.
Eyal Karzbrun 1 2,  Rami Yair Tshuva 1,  Orly Reiner 1
Affiliations

Affiliations

  • 1 Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
  • 2 Department of Physics and the Kavli Institute for Theoretical Physics, University of California Santa Barbara, Santa Barbara, California.
PMID: 30239150 DOI: 10.1002/cpcb.62
Abstract

Brain organoids are an emerging technique for studying human neurodevelopment in vitro, with biomedical implications. However, three-dimensional tissue culture poses several challenges, including lack of nutrient exchange at the organoid core and limited imaging accessibility of whole organoids. Here we present a method for culturing organoids in a micro-fabricated device that enables in situ real-time imaging over weeks with efficient nutrient exchange by diffusion. Our on-chip approach offers a means for studying the dynamics of organoid development, cell differentiation, cell cycle, and motion. © 2018 by John Wiley & Sons, Inc.

Keywords

3D cell culture; brain organoids; human pluripotent stem cells; lab on a chip; microfabrication; neurodevelopment.

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