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  4. 5-Carboxyfluorescein diacetate N-succinimidyl ester

5-Carboxyfluorescein diacetate N-succinimidyl ester 

Cat. No.: HY-D0056 Purity: 99.50%
COA Handling Instructions

Molarity Calculator

5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation.

For research use only. We do not sell to patients.

5-Carboxyfluorescein diacetate N-succinimidyl ester Chemical Structure

5-Carboxyfluorescein diacetate N-succinimidyl ester Chemical Structure

CAS No. : 150206-05-6

Size Price Stock Quantity
5 mg USD 75 In-stock
10 mg USD 120 In-stock
25 mg USD 220 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Based on 5 publication(s) in Google Scholar

5-Carboxyfluorescein diacetate N-succinimidyl ester Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dilute the stock solution in PBS (to give a 50 μM solution).
2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly.
3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS.
4. Centrifuge cells 5 min at 300 ×g, 20 ℃.
5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS.
6. Centrifuge cells 5 min at 300 × g, 20 ℃, and remove the supernatant.
Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-Carboxyfluorescein diacetate N-succinimidyl ester Related Antibodies
Molecular Weight

557.46

Formula

C29H19NO11
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

525
Excitation (Ex)

482
SMILES

O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)ON6C(CCC6=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (37.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7939 mL 8.9693 mL 17.9385 mL 44.8463 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL 8.9693 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL 4.4846 mL
15 mM 0.1196 mL 0.5980 mL 1.1959 mL 2.9898 mL
20 mM 0.0897 mL 0.4485 mL 0.8969 mL 2.2423 mL
25 mM 0.0718 mL 0.3588 mL 0.7175 mL 1.7939 mL
30 mM 0.0598 mL 0.2990 mL 0.5980 mL 1.4949 mL
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5-Carboxyfluorescein diacetate N-succinimidyl ester Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Carboxyfluorescein diacetate N-succinimidyl ester150206-05-6Fluorescent DyeDyelymphocytemigrationproliferationCFSEInhibitorinhibitorinhibit

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