BML-284

Name: BML-284
Brand: MedChemExpress
SKU: HY-19987
Rating: 5.0 (41 reviews)

Cat. No.: HY-19987 Purity: 99.99%: Data Sheet
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BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC₅₀ of 700 nM.

For research use only. We do not sell to patients.

CAS No. : 853220-52-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Customer Review

Based on 41 publication(s) in Google Scholar

Other Forms of BML-284:

BML-284 hydrochloride Get quote
BML-284 (Standard) Get quote

41 Publications Citing Use of MCE BML-284

Cell Imaging/Staining

RT-PCR

In Vivo Efficacy Study

: BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]; Protein level of β-catenin, PPARγ, and GAPDH for RCT-FAPs (FAPs isolated from supraspinatus muscle one week after tendon tears) with or without β-catenin agonist BML-284 (1 μM) treatment.

: BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]; The oil red staining and statistical analysis of RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).

: BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]; Relative mRNA expression level of adipogenic genes for RCT-FAPs after 7-day adipogenic induction in AIM with DMSO or BML-284 (1 μM).

: BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]; Immunofluorescence staining of perilipin1 for supraspinatus muscles from the DMSO group and BML-284 (1 μM) group.

: BML-284 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Aug 25;9(1):312. [Abstract]; Gross appearance of supraspinatus muscles from the DMSO and BML-284 (1 μM) treatment groups.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

BML-284 is a potent and cell-permeable Wnt signaling activator. BML-284 induces TCF-dependent transcriptional activity with an EC₅₀ of 700 nM^[1].

IC₅₀ & Target

EC50: 700 nM (TCF-dependent transcriptional activity)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.52 μM Compound: BML284	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
HeLa	IC₅₀	0.09 μM Compound: BML284	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human HeLa cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
K562	IC₅₀	0.1 μM Compound: BML284	Antiproliferative activity against human K562 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human K562 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
MCF7	IC₅₀	0.69 μM Compound: BML284	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]
MOLM-13	IC₅₀	0.19 μM Compound: BML284	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 72 hrs Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 72 hrs	[PMID: 38430854]

In Vitro

BML-284 (10 μM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen (HY-B0115)^[1].
BML-284 (10 μM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	Human gastric cancer cell lines MNK45 and AGS^[1]
Concentration:	10 µM
Incubation Time:	24 hours
Result:	Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.

In Vivo

BML-284 (10 ng) combines with Pyrimethanil (4 mg/L) could partially rescue the malformed phenotype and cardiac defects induced by Pyrimethanil in Tg (myl7:EGFP) transgenic embryos at 5.5 hpf are transferred into plates with 20 embryos^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

350.37

Formula

C₁₉H₁₈N₄O₃

CAS No.

853220-52-7

Appearance

Solid

Color

White to yellow

SMILES

NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (285.41 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8541 mL	14.2706 mL	28.5413 mL
5 mM	0.5708 mL	2.8541 mL	5.7083 mL
10 mM	0.2854 mL	1.4271 mL	2.8541 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (246 KB) HNMR (279 KB) RP-HPLC (247 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Liu J, et al. A small-molecule agonist of the Wnt signaling pathway. Angew Chem Int Ed Engl. 2005 Mar 18;44(13):1987-90. [Content Brief]

[2]. Ying Jiang, et al. Pizotifen inhibits the proliferation and invasion of gastric cancer cells. Exp Ther Med. 2020 Feb;19(2):817-824. [Content Brief]

[3]. Yunlong Meng, et al. Exposure to pyrimethanil induces developmental toxicity and cardiotoxicity in zebrafish. Chemosphere. 2020 Sep;255:126889. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8541 mL	14.2706 mL	28.5413 mL	71.3531 mL
	5 mM	0.5708 mL	2.8541 mL	5.7083 mL	14.2706 mL
	10 mM	0.2854 mL	1.4271 mL	2.8541 mL	7.1353 mL
	15 mM	0.1903 mL	0.9514 mL	1.9028 mL	4.7569 mL
	20 mM	0.1427 mL	0.7135 mL	1.4271 mL	3.5677 mL
	25 mM	0.1142 mL	0.5708 mL	1.1417 mL	2.8541 mL
	30 mM	0.0951 mL	0.4757 mL	0.9514 mL	2.3784 mL
	40 mM	0.0714 mL	0.3568 mL	0.7135 mL	1.7838 mL
	50 mM	0.0571 mL	0.2854 mL	0.5708 mL	1.4271 mL
	60 mM	0.0476 mL	0.2378 mL	0.4757 mL	1.1892 mL
	80 mM	0.0357 mL	0.1784 mL	0.3568 mL	0.8919 mL
	100 mM	0.0285 mL	0.1427 mL	0.2854 mL	0.7135 mL

BML-284 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BML-284853220-52-7BML284BML 284WntTCFMNK45AGSE-cadherinN-cadherinmalformed phenotypecardiac defectInhibitorinhibitorinhibit

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BML-284

Customer Review

Other Forms of BML-284:

BML-284 Related Antibodies

41 Publications Citing Use of MCE BML-284

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Wnt Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

BML-284 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

BML-284 Related Classifications

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