Dansylcadaverine (Synonyms: Monodansyl cadaverine)

Name: Dansylcadaverine
Brand: MedChemExpress
SKU: HY-D1027
Rating: 5.0 (5 reviews)

Cat. No.: HY-D1027 Purity: 98.62%: FAQs
Data Sheet SDS COA Handling Instructions

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Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.

For research use only. We do not sell to patients.

Dansylcadaverine Chemical Structure

CAS No. : 10121-91-2

Size	Price	Stock	Quantity
50 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

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Based on 5 publication(s) in Google Scholar

5 Publications Citing Use of MCE Dansylcadaverine

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Description

In Vitro

The inhibitory activity of dansylcadaverine reflects its ability to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules^[2].
? Dansylcadaverine, a cationic fluorescent probe binds to bacterial lipopolysaccharide and lipid A, and is displaced competitively by other compounds which possess affinity toward endotoxins^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

335.46

Formula

C₁₇H₂₅N₃O₂S

CAS No.

10121-91-2

Appearance

Solid

Color

White to off-white

SMILES

NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9810 mL	14.9049 mL	29.8098 mL
5 mM	0.5962 mL	2.9810 mL	5.9620 mL
10 mM	0.2981 mL	1.4905 mL	2.9810 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light, stored under nitrogen). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.62%

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Data Sheet (276 KB) SDS (0 KB)

COA (247 KB) HNMR (229 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

Dyeing Example

References

[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [Content Brief]

[2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9. [Content Brief]

[3]. Gao L, et al. Autophagy blockade sensitizes human head and neck squamous cell carcinoma towards CYT997 through enhancing excessively high reactive oxygen species-induced apoptosis. J Mol Med (Berl). 2018;96(9):929-938. [Content Brief]

[4]. Cornwell MM, et al. Inhibition of the adhesion of Chinese hamster ovary cells by the naphthylsulfonamides dansylcadaverine and N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide (W7). Biochim Biophys Acta. 1983;762(3):414-419. [Content Brief]

[5]. David SA, et al. Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim Biophys Acta. 1992;1165(2):147-152. [Content Brief]

Kinase Assay ^[1]	To determine the time course of transglutamination, thymosin β₄ (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl₂, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay	MCF7 cells (2.4×10⁴) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20. [Content Brief] [2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9. [Content Brief] [3]. Gao L, et al. Autophagy blockade sensitizes human head and neck squamous cell carcinoma towards CYT997 through enhancing excessively high reactive oxygen species-induced apoptosis. J Mol Med (Berl). 2018;96(9):929-938. [Content Brief] [4]. Cornwell MM, et al. Inhibition of the adhesion of Chinese hamster ovary cells by the naphthylsulfonamides dansylcadaverine and N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide (W7). Biochim Biophys Acta. 1983;762(3):414-419. [Content Brief] [5]. David SA, et al. Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim Biophys Acta. 1992;1165(2):147-152. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9810 mL	14.9049 mL	29.8098 mL	74.5245 mL
	5 mM	0.5962 mL	2.9810 mL	5.9620 mL	14.9049 mL
	10 mM	0.2981 mL	1.4905 mL	2.9810 mL	7.4525 mL
	15 mM	0.1987 mL	0.9937 mL	1.9873 mL	4.9683 mL
	20 mM	0.1490 mL	0.7452 mL	1.4905 mL	3.7262 mL
	25 mM	0.1192 mL	0.5962 mL	1.1924 mL	2.9810 mL
	30 mM	0.0994 mL	0.4968 mL	0.9937 mL	2.4842 mL
	40 mM	0.0745 mL	0.3726 mL	0.7452 mL	1.8631 mL
	50 mM	0.0596 mL	0.2981 mL	0.5962 mL	1.4905 mL
	60 mM	0.0497 mL	0.2484 mL	0.4968 mL	1.2421 mL
	80 mM	0.0373 mL	0.1863 mL	0.3726 mL	0.9316 mL
	100 mM	0.0298 mL	0.1490 mL	0.2981 mL	0.7452 mL

Dansylcadaverine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dansylcadaverine10121-91-2Monodansyl cadaverineAutophagyautofluorescentprobeautophagicvacuolessubstrateInhibitorinhibitorinhibit

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Dansylcadaverine (Synonyms: Monodansyl cadaverine)

Customer Review

Dansylcadaverine Related Antibodies

5 Publications Citing Use of MCE Dansylcadaverine

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Dansylcadaverine Related Antibodies

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Complete Stock Solution Preparation Table

Dansylcadaverine Related Classifications

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