EGF Receptor Substrate 2 (Phospho-Tyr5)

Name: EGF Receptor Substrate 2 (Phospho-Tyr5)
Brand: MedChemExpress
SKU: HY-P0320
Rating: 4.5 (1 reviews)

Cat. No.: HY-P0320 Purity: 99.53%: FAQs
Data Sheet SDS COA Handling Instructions

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EGF Receptor Substrate 2 (Phospho-Tyr5) acetate, a biologically active peptide, is a tyrosine phosphate substrate. EGF Receptor Substrate 2 (Phospho-Tyr5) acetate can be used to detect protein tyrosine phosphatases activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

EGF Receptor Substrate 2 (Phospho-Tyr5) Chemical Structure

CAS No. : 149261-42-7

Size	Price	Stock	Quantity
1 mg	USD 108	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 312	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 552	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of EGF Receptor Substrate 2 (Phospho-Tyr5):

EGF Receptor Substrate 2 (Phospho-Tyr5) (TFA) Get quote
EGF Receptor Substrate 2 (Phospho-Tyr5) (acetate) Get quote

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Molecular Weight

1328.28

Formula

C₅₄H₈₂N₁₃O₂₄P

CAS No.

149261-42-7

Appearance

Solid

Color

White to off-white

Sequence

H-Asp-Ala-Asp-Glu-{pTyr}-Leu-Ile-Pro-Gln-Gln-Gly

Sequence Shortening

DADE-{pTyr}-LIPQQG

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (37.64 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7529 mL	3.7643 mL	7.5285 mL
5 mM	0.1506 mL	0.7529 mL	1.5057 mL
10 mM	0.0753 mL	0.3764 mL	0.7529 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Dosing volume
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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.53%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (253 KB) LCMS (268 KB) Elemental Analysis Report (113 KB)

Handling Instructions (2659 KB)

References

[1]. Kuban-Jankowska A, et al. Inactivation of Protein Tyrosine Phosphatases by Peracids Correlates with the Hydrocarbon Chain Length. Cell Physiol Biochem. 2015;36(3):1069-83. [Content Brief]

Kinase Assay
^[1]

Briefly, the assays are performed in 50 μL volumes in a microtiter plate using the generalized PTPase substrates ENDpYINASL and DADEpYLIPQQG (where pY represents phosphotyrosine) and assay buffer containing 25 mM Tris-HCl (pH 7.4), 50 mM NaCl, 2 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 1 mg of bovine serum albumin/mL. The reactions are started by the addition of 5 μg of either CAV GST-VP2, GST, CAV GST-VP2 containing the C95S mutation, or TLMV GST-ORF2 or 2 units of the positive control T cell protein-tyrosine phosphatase (TC-PTP) in assay buffer. Control reactions are assayed with either ENDpYINASL substrate alone, DADEpYLIPQQG substrate alone, CAV GST-VP2 without substrate, CAV GST-VP2 containing the C95S mutation without substrate, TLMV GST-ORF2 without substrate, TC-PTP without substrate, or assay buffer with neither enzyme nor substrate. A phosphate standard curve is derived using a supplied phosphate standard. The reactions are terminated by the addition of the malachite green detection reagent.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Kuban-Jankowska A, et al. Inactivation of Protein Tyrosine Phosphatases by Peracids Correlates with the Hydrocarbon Chain Length. Cell Physiol Biochem. 2015;36(3):1069-83. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.7529 mL	3.7643 mL	7.5285 mL	18.8213 mL
	5 mM	0.1506 mL	0.7529 mL	1.5057 mL	3.7643 mL
	10 mM	0.0753 mL	0.3764 mL	0.7529 mL	1.8821 mL
	15 mM	0.0502 mL	0.2510 mL	0.5019 mL	1.2548 mL
	20 mM	0.0376 mL	0.1882 mL	0.3764 mL	0.9411 mL
	25 mM	0.0301 mL	0.1506 mL	0.3011 mL	0.7529 mL
	30 mM	0.0251 mL	0.1255 mL	0.2510 mL	0.6274 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.