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  4. HOE-S 785026 trihydrochloride

HOE-S 785026 trihydrochloride  (Synonyms: meta-Hoechst trihydrochloride)

Cat. No.: HY-15561B Purity: 99.76%
COA Handling Instructions

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HOE-S 785026 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

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HOE-S 785026 trihydrochloride Chemical Structure

HOE-S 785026 trihydrochloride Chemical Structure

CAS No. : 2320308-12-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 341 In-stock
Solution
10 mM * 1 mL in Water USD 341 In-stock
Solid
5 mg USD 290 In-stock
10 mg USD 450 In-stock
25 mg USD 810 In-stock
50 mg USD 1130 In-stock
100 mg USD 1580 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of HOE-S 785026 trihydrochloride:

HOE-S 785026 trihydrochloride Related Antibodies

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Description

HOE-S 785026 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

In Vitro

General Protocol
Preparation of Hoechst working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of in 5 mL DMSO
Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
1.Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Precautions
1. Please adjust the concentration of Hoechst working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HOE-S 785026 trihydrochloride Related Antibodies
Molecular Weight

533.88

Formula

C25H27Cl3N6O
CAS No.
Appearance

Solid

Color

Light green to green

Emission (Em)

451
Excitation (Ex)

356
SMILES

OC1=CC=CC(C2=NC3=CC(C4=NC5=CC(N6CCN(C)CC6)=CC=C5N4)=CC=C3N2)=C1.[H]Cl.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 47.3 mg/mL (88.60 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8731 mL 9.3654 mL 18.7308 mL
5 mM 0.3746 mL 1.8731 mL 3.7462 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.8731 mL 9.3654 mL 18.7308 mL 46.8270 mL
5 mM 0.3746 mL 1.8731 mL 3.7462 mL 9.3654 mL
10 mM 0.1873 mL 0.9365 mL 1.8731 mL 4.6827 mL
15 mM 0.1249 mL 0.6244 mL 1.2487 mL 3.1218 mL
20 mM 0.0937 mL 0.4683 mL 0.9365 mL 2.3414 mL
25 mM 0.0749 mL 0.3746 mL 0.7492 mL 1.8731 mL
30 mM 0.0624 mL 0.3122 mL 0.6244 mL 1.5609 mL
40 mM 0.0468 mL 0.2341 mL 0.4683 mL 1.1707 mL
50 mM 0.0375 mL 0.1873 mL 0.3746 mL 0.9365 mL
60 mM 0.0312 mL 0.1561 mL 0.3122 mL 0.7805 mL
80 mM 0.0234 mL 0.1171 mL 0.2341 mL 0.5853 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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HOE-S 785026 trihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HOE-S 785026 trihydrochloride2320308-12-9meta-Hoechst trihydrochlorideFluorescent DyeHoechstDNAcellular dyeblue fluorescenceInhibitorinhibitorinhibit

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