1. Antibodies
  2. Primary Antibodies
  3. Caspase-14 Antibody

Caspase-14 Antibody

Cat. No.: HY-P80045
COA User Guide for Antibodies

Caspase-14 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 28 kDa, targeting to Caspase-14. It can be used for WB,ICC/IF,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Caspase-14 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 28 kDa, targeting to Caspase-14. It can be used for WB,ICC/IF,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse.

Tag

Free

Molecular Weight

Predicted band size: 28 kDa

Purity

Protein A affinity purified.

Subcellular Location

Cytoplasm, Nucleus.

Conjugation

Non-conjugated

Research Field

Cell Biology

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse

SwissProt ID

SwissProt: P31944 Human;SwissProt: O89094 Mouse;Unigene: 228179 Rat

Dilution Ratio

WB: 1:500-1:2,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

  • Western blot analysis of extracts from MCF-7(lane 2(20μg) and MCF-7(lane 3(40μg), HepG2(lane 4(20μg) ) and HepG2(lane 5(40μg) ) using Caspase-14 (HY-P80045) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80045, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application

WB, ICC/IF, IHC-P, FC, IP

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Immunogen

Synthetic peptide corresponding to Human Caspase-14.AA range:80-220.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Caspase-14 Antibody
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HY-P80045
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