Doxycycline calcium 

Doxycycline calcium, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor^[1]. Doxycycline calcium shows antibacterial activity and anti-cancer cell proliferation activity^{[1][2][3][4][5]}.

Doxycycline calcium (0.01-10 µg/mL, 4 d) affects growth of glioma cells only under high concentrations^[2].
Doxycycline calcium (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells^[2].
Doxycycline calcium (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines^[4].
Doxycycline calcium (0-250 μM, 72 h) inhibits cell viability of breast cancer cells ^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Doxycycline (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner^[3].
Doxycycline and Tetracycline (HY-A0107), act systemically after absorption from the upper gastrointestinal tract. The main advantage of Doxycycline over Tetracycline is its longer activity, and it can be taken twice or once a day. The peak concentration of both drugs is similar, but in the case of Doxycycline the time to peak concentration is shorter, and half life is significantly longer^[6].

Doxycycline (Dox) is often used as an inducer in molecular biology studies to induce gene expression. In cells or model animals that have constructed tetracycline induced expression systems (Tet-On/Tet-Off systems), the expression of target genes can be precisely controlled by adding or removing Dox^{[7][8][9][10]}.

Dose reference for Dox induction^{[7][8][9][10]}:
(1) Model animal: male Sprague–Dawley rats
Tet regulatory system：20-3000 ppm of Dox is supplied in diet
(2) Model animal: Cags mice
Tet regulatory system：625 ppm of Dox is supplied in diet
(3) Model animal: Transgenic Wistar rats
Tet regulatory system：2 mg/mL of Dox is supplied in drinking water
(4) Model animal: Transgenic NRMI inbred mice
Tet regulatory system：2 mg/mL of Dox is supplied in drinking water

Dissolution method of Dox^[9][10]:
(1) Prepare Dox working solution
Dissolve 100 mg Dox into 50 mL drinking water, add 5% sucrose or 2% saccharin to mask the bitter taste, and refresh the water every three days.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

524.59

C₂₂H₂₄Ca₂N₂O₈

94088-85-4

O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@@H](C)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.[Ca].[Ca]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Eusebio Manchado, et al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016 Jun 30;534(7609):647-51.  [Content Brief]

  [2]. Anna-Luisa Luger, et al. Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci. 2018 May 17;19(5):1504.  [Content Brief]

  [3]. Wilfried Briest, et al. Doxycycline ameliorates the susceptibility to aortic lesions in a mouse model for the vascular type of Ehlers-Danlos syndrome. J Pharmacol Exp Ther. 2011 Jun;337(3):621-7.  [Content Brief]

  [4]. Ethan Ahler, et al. Doxycycline alters metabolism and proliferation of human cell lines. PLoS One. 2013 May 31;8(5):e64561.  [Content Brief]

  [5]. Le Zhang, et al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017 Apr 18;16(8):737-745.  [Content Brief]

  [6]. Niv Y. Doxycycline in Eradication Therapy of Helicobacter pylori--a Systematic Review and Meta-Analysis. Digestion. 2016;93(2):167-73.  [Content Brief]

  [7]. Manfredsson FP, et al. Tight Long-term dynamic doxycycline responsive nigrostriatal GDNF using a single rAAV vector. Mol Ther. 2009 Nov;17(11):1857-67.  [Content Brief]

  [8]. Redelsperger IM, et al. Stability of Doxycycline in Feed and Water and Minimal Effective Doses in Tetracycline-Inducible Systems. J Am Assoc Lab Anim Sci. 2016;55(4):467-74.  [Content Brief]

  [9]. Konopka W, et al. Tet system in the brain: transgenic rats and lentiviral vectors approach. Genesis. 2009 Apr;47(4):274-80.  [Content Brief]

  [10]. Kistner A, et al. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10933-8.  [Content Brief]