2. Apoptosis
  3. Apoptosis
  4. IMM-01

IMM-01 is a formin agonist that inhibits DID-DAD (diaphanous inhibitory domain-diaphanous autoregulatory domain) binding with an IC50 140 nM. IMM-01 acts by disrupting the autoinhibitory bond between the DID and DAD domain and thus activates formins. IMM-01 shows anticancer effects.

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IMM-01 Chemical Structure

IMM-01 Chemical Structure

CAS No. : 218795-74-5

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Based on 1 publication(s) in Google Scholar

IMM-01 Related Antibodies

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Description

IMM-01 is a formin agonist that inhibits DID-DAD (diaphanous inhibitory domain-diaphanous autoregulatory domain) binding with an IC50 140 nM. IMM-01 acts by disrupting the autoinhibitory bond between the DID and DAD domain and thus activates formins. IMM-01 shows anticancer effects[1].

In Vitro

IMM-01 (100 μM; 1h) induces microtubule stabilization in SW480 colon carcinoma cells[1].
IMM-01 (10 μM) significantly induces LacZ expression in NIH 3T3-SRE-LacZ cells[1].
IMM-01 (1-100 μM; 48 hours) induces caspase-3 cleavage during induction of apoptosis in NIH 3T3 cells and SW480 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IMM-01 Related Antibodies

Apoptosis Analysis[1]

Cell Line: NIH 3T3 cells and SW480 cells
Concentration: 1 μM, 10 μM, or 100 μM
Incubation Time: 48 hours
Result: Induced caspase-3 cleavage.
In Vivo

IMM-01 (5-25 mg/kg; i.v.; 2 times a week; for 3 weeks) is able to slow tumor growth in a mouse xenograft model of colon cancer[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude female mice (6 to 8 weeks) implanted with SW480 cells[1]
Dosage: 5 mg/kg, 25 mg/kg
Administration: i.v.; 2 times a week; for 3 weeks
Result: Slowed tumor growth in a dose-dependent manner when administered intravenously via the tail vein.
Clinical Trial
Molecular Weight

267.35

Formula

C12H17N3O2S
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

OC1=CC=C(/C=N/NC(NC(C)(C)C)=S)C(O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (467.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7404 mL 18.7021 mL 37.4041 mL
5 mM 0.7481 mL 3.7404 mL 7.4808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7404 mL 18.7021 mL 37.4042 mL 93.5104 mL
5 mM 0.7481 mL 3.7404 mL 7.4808 mL 18.7021 mL
10 mM 0.3740 mL 1.8702 mL 3.7404 mL 9.3510 mL
15 mM 0.2494 mL 1.2468 mL 2.4936 mL 6.2340 mL
20 mM 0.1870 mL 0.9351 mL 1.8702 mL 4.6755 mL
25 mM 0.1496 mL 0.7481 mL 1.4962 mL 3.7404 mL
30 mM 0.1247 mL 0.6234 mL 1.2468 mL 3.1170 mL
40 mM 0.0935 mL 0.4676 mL 0.9351 mL 2.3378 mL
50 mM 0.0748 mL 0.3740 mL 0.7481 mL 1.8702 mL
60 mM 0.0623 mL 0.3117 mL 0.6234 mL 1.5585 mL
80 mM 0.0468 mL 0.2338 mL 0.4676 mL 1.1689 mL
100 mM 0.0374 mL 0.1870 mL 0.3740 mL 0.9351 mL
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IMM-01 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IMM-01218795-74-5IMM01IMM 01Apoptosisformin agonistDID-DADanticancer effectscaspase-3Inhibitorinhibitorinhibit

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