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  5. Deacetylase Inhibitor Cocktail (100× in 70% DMSO)

Deacetylase Inhibitor Cocktail (100× in 70% DMSO)  (100× in 70% DMSO)

Cat. No.: HY-K0030
Manual SDS

Deacetylase Inhibitor Cocktail is a synergistic combination of chemicals designed to preserve the acetylation state of proteins.

Deacetylase Inhibitor Cocktail  (100× in 70% DMSO)
Size (Pre-dissolved in DMSO) Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 45 In-stock
10 mL USD 200 In-stock
50 mL USD 800 In-stock
100 mL USD 1280 In-stock

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Deacetylase Inhibitor Cocktail is a synergistic combination of chemicals designed to preserve the acetylation state of proteins. Lysine acetylation is an important post-translational modification that can affect protein transcription, cell cycle, and signal transduction pathways. The balance between acetyltransferase enzymes and deacetylase enzymes affects chromatin condensation, transcription factor activity and protein aggregation and is of significant interest to researchers. Deacetylation Inhibition Cocktail is a useful tool for investigating these important biological pathways.

MedChemExpress Deacetylase Inhibitor Cocktail (100× in 70% DMSO) is provided as 1 mL vial. Each vial contains 1 mL deacetylase inhibitor cocktail solubilized in 70% DMSO with the following components: Trichostatin A (40 μM), EX-527 (1 mM), Nicotinamide (400 mM), and Sodium Butyrate (200 mM). Each component has specific inhibitory properties. Trichostatin A acts to inhibit class I/II HDAC. EX-527 inhibits SIRT. Nicotinamide acts against class III HDAC. Sodium Butyrate acts against class I/II HDAC.

Storage

Deacetylase Inhibitor Cocktail (100× in 70% DMSO) can be stored at -20°C for 12 months.

Protocol

1. This product can be applied in Western Blotting, Flow Cytometry, and ImmunoFluorescence and etc.

2. Thaw at room temperature, add at 1:100 (v/v) dilution to culture media, recommended incubation times of 16-48 hours, before assaying.

3. One tube of Protease Inhibitor Cocktail is recommended for inhibition of proteases present in cell for Western Blotting.

Components
Ingredient M.Wt 100× Conc. Target
Trichostatin A 302.37 40 μM Class I/II HDAC
EX-527 248.71 1 mM SIRT
Nicotinamide 122.12 400 mM Class III HDAC
Sodium Butyrate 110.09 200 mM Class I/II HDAC
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Deacetylase Inhibitor Cocktail (100× in 70% DMSO)
Cat. No.:
HY-K0030
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