1. MCE Kits
  2. SYBR Green qPCR Master Mix

SYBR Green qPCR Master Mix 

Cat. No.: HY-K0501
Manual SDS

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.

SYBR Green qPCR Master Mix
Size Price Stock Quantity
1 mL (100 rxns) $55 In-stock
5 mL (500 rxns) $185 In-stock
20 mL (2000 rxns) $665 In-stock

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Customer Validation

mRNA expression of AB, SO, CD, OC, NA and GP genes in H-GFP and H-5A cell lines assessed by RT-qPCR.

Relative expression of MDM2, p21, Survivin, PUMA, BAX and Bcl2 genes in N-3a treated cells compared to controls.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells.

 

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Passive reference dyes are provided for different qPCR instruments.

Storage

Stored at -20°C protecting from light, and is stable for up to 18 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.

Components
Components HY-K0501-1 mL (100 rxn) HY-K0501-5 mL (500 rxn) HY-K0501-20 mL (2000 rxn)
SYBR Greenq PCR Master Mix (2×) 1 mL 1 mL × 5 1 mL × 20
ROX Reference Dye 40 μL 200 μL 800 μL
ROX Reference Dye II 40 μL 200 μL 800 μL
Documentation

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Product Name:
SYBR Green qPCR Master Mix
Cat. No.:
HY-K0501
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