2. Academic Validation
  3. Differentiation-inducing action of 10-hydroxycamptothecin on human hepatoma Hep G2 cells

Differentiation-inducing action of 10-hydroxycamptothecin on human hepatoma Hep G2 cells

  • Acta Pharmacol Sin. 2000 Apr;21(4):364-8.
X W Zhang 1,  J F Jiang,  B Xu
Affiliations

Affiliation

  • 1 Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
PMID: 11324469
Abstract

Aim: To study the mechanism of differentiation-inducing action of 10-hydroxycamptothecin (HCPT) on human hepatoma Hep G2 cells.

Methods: The proliferating cell nuclear antigen (PCNA) expression was studied by immunocytochemical staining method. The cell cycle distribution and the wild-type protein p53 expression were measured by flow cytometry. Telomerase activity was assayed with telomeric repeat amplification protocol (TRAP).

Results: After treatment with HCPT at differentiation-inducing concentrations 5-20 micrograms.L-1 for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 micrograms.L-1 for 6 d, the p53 expression level markedly increased in comparison with the control cells. The Telomerase activity did not change in Hep G2 cells treated with HCPT 5-20 micrograms.L-1 for 8 d.

Conclusion: The differentiation-inducing effect of HCPT on Hep G2 cells is related with the cell cycle arrest at G2/M phase, down-regulation of PCNA and up-regulation of wild-type protein p53.

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