2. Academic Validation
  3. PACAP 1-38 as neurotransmitter in the porcine antrum

PACAP 1-38 as neurotransmitter in the porcine antrum

  • Regul Pept. 2001 Sep 15;101(1-3):109-21. doi: 10.1016/s0167-0115(01)00276-2.
K Tornøe 1,  J Hannibal,  B Georg,  P T Schmidt,  L Hilsted,  J Fahrenkrug,  J J Holst
Affiliations

Affiliation

  • 1 Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, Dk-2200, N, Copenhagen, Denmark.
PMID: 11495686 DOI: 10.1016/s0167-0115(01)00276-2
Abstract

The concentration of PACAP 1-38 in porcine antrum amounted to 15.4+/-7.9 and 20.3+/-8 pmol/g tissue in the mucosal and muscular layers. PACAP immunoreactive (IR) fibres innervated the muscular (co-localised with VIP) and submucosal/mucosal layers (some co-storing VIP and CGRP) including myenteric and submucosal plexus and blood vessels. Only myenteric nerve cell bodies contained PACAP-IR (co-storing VIP). In isolated perfused antrum, vagus nerve stimulation (8 Hz) and capsaicin (10(-5) M) increased PACAP 1-38 release. PACAP 1-38 (10(-9) M) increased substance P (SP), Gastrin releasing peptide (GRP) and VIP release. PACAP 1-38 (10(-8) M) inhibited Gastrin secretion and stimulated somatostatin secretion and motility dose-dependently. PACAP-induced motility was strongly inhibited by the antagonist PACAP 6-38 but also by atropine and substance P-antagonists (CP99994/SR48968) but PACAP 6-38 had no effect on vagus-induced secretion or motility.

Conclusion: PACAP 1-38 may be involved in antral motility and secretion by interacting with cholinergic, SP-ergic, GRP-ergic and/or VIP-ergic neurones, and may also be involved in afferent reflex pathways.

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