2. Academic Validation
  3. Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression

Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression

  • Cytokine. 2015 Dec;76(2):206-213. doi: 10.1016/j.cyto.2015.05.016.
Vengadeshprabhu Karuppagounder 1,  Somasundaram Arumugam 1,  Rajarajan Amirthalingam Thandavarayan 2,  Vigneshwaran Pitchaimani 1,  Remya Sreedhar 1,  Rejina Afrin 1,  Meilei Harima 1,  Hiroshi Suzuki 1,  Mayumi Nomoto 1,  Shizuka Miyashita 1,  Kenji Suzuki 3,  Masahiko Nakamura 4,  Kazuyuki Ueno 5,  Kenichi Watanabe 6
Affiliations

Affiliations

  • 1 Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan.
  • 2 Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan; Department of Cardiovascular Sciences, Houston Methodist Research Institute, Houston, TX 77030, USA.
  • 3 Department of Gastroenterology, Niigata University Graduate School of Medical and Dental Sciences, Niigata City 951-8510, Japan.
  • 4 Department of Cardiology, Yamanashi Prefectural Center Hospital, 1-1-1 Fujimi Kofu, Yamanashi 400-8506, Japan.
  • 5 Department of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan.
  • 6 Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan. Electronic address: [email protected].
PMID: 26049169 DOI: 10.1016/j.cyto.2015.05.016
Abstract

Polyphenolic compound tannic acid, which is mainly found in grapes and green tea, is a potent antioxidant with anticarcinogenic activities. In this present study, we hypothesized that tannic acid could inhibit nuclear factor (NF)κB signaling and inflammation in atopic dermatitis (AD) NC/Nga mice. We have analyzed the effects of tannic acid on dermatitis severity, histopathology and expression of inflammatory signaling proteins in house dust Mite extract induced AD mouse skin. In addition, serum levels of T helper (Th) cytokines (interferon (IFN)γ, interleukin (IL)-4) were measured by enzyme-linked immunosorbent assay. Treatment with tannic acid ameliorated the development of AD-like clinical symptoms and effectively inhibited hyperkeratosis, parakeratosis, acanthosis, mast cells and infiltration of inflammatory cells in the AD mouse skin. Serum levels of IFNγ and IL-4 were significantly down-regulated by tannic acid. Furthermore, tannic acid treatment inhibited DfE induced tumor necrosis factor (TNF)α, high mobility group protein (HMG)B1, receptor for advanced glycation end products (RAGE), extracellular signal-regulated kinase (ERK)1/2, NFκB, cyclooxygenase (COX)2, IL-1β and increased the protein expression of Peroxisome Proliferator-activated Receptor (PPAR)γ. Taken together, our results demonstrate that, DfE induced skin inflammation might be mediated through NFκB signaling and tannic acid may be a potential therapeutic agent for AD, which may possibly act via induction of PPARγ protein.

Keywords

Cytokine; Nuclear factor kappa B; Peroxisome proliferator-activated receptor; Tannic acid; Tumor necrosis factor.

Figures
Products