2. Academic Validation
  3. FH535 inhibited metastasis and growth of pancreatic cancer cells

FH535 inhibited metastasis and growth of pancreatic cancer cells

  • Onco Targets Ther. 2015 Jul 6;8:1651-70. doi: 10.2147/OTT.S82718.
Meng-Yao Wu 1,  Rong-Rui Liang 1,  Kai Chen 1,  Meng Shen 1,  Ya-Li Tian 2,  Dao-Ming Li 1,  Wei-Ming Duan 1,  Qi Gui 1,  Fei-Ran Gong 3,  Lian Lian 2,  Wei Li 4,  Min Tao 5
Affiliations

Affiliations

  • 1 Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou Xiangcheng People's Hospital, Suzhou, People's Republic of China.
  • 2 Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou Xiangcheng People's Hospital, Suzhou, People's Republic of China ; Department of Oncology, Suzhou Xiangcheng People's Hospital, Suzhou, People's Republic of China.
  • 3 Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China.
  • 4 Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou Xiangcheng People's Hospital, Suzhou, People's Republic of China ; PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou, People's Republic of China.
  • 5 Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou Xiangcheng People's Hospital, Suzhou, People's Republic of China ; Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou, People's Republic of China ; Institute of Medical Biotechnology, Soochow University, Suzhou, People's Republic of China ; PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou, People's Republic of China.
PMID: 26185454 DOI: 10.2147/OTT.S82718
Abstract

FH535 is a small-molecule inhibitor of the Wnt/β-catenin signaling pathway, which a substantial body of evidence has proven is activated in various cancers, including pancreatic Cancer. Activation of the Wnt/β-catenin pathway plays an important role in tumor progression and metastasis. We investigated the inhibitory effect of FH535 on the metastasis and growth of pancreatic Cancer cells. Western blotting and luciferase reporter gene assay indicated that FH535 markedly inhibited Wnt/β-catenin pathway viability in pancreatic Cancer cells. In vitro wound healing, invasion, and adhesion assays revealed that FH535 significantly inhibited pancreatic Cancer cell metastasis. We also observed the inhibitory effect of FH535 on pancreatic Cancer cell growth via the tetrazolium and plate clone formation assays. Microarray analyses suggested that changes in the expression of multiple genes could be involved in the anti-cancer effect of FH535 on pancreatic Cancer cells. Our results indicate for the first time that FH535 inhibits pancreatic Cancer cell metastasis and growth, providing new insight into therapy of pancreatic Cancer.

Keywords

FH535; growth; metastasis; pancreatic cancer; β-catenin.

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