2. Academic Validation
  3. Cytotoxicity of Aconitum alkaloid and its interaction with calf thymus DNA by multi-spectroscopic techniques

Cytotoxicity of Aconitum alkaloid and its interaction with calf thymus DNA by multi-spectroscopic techniques

  • Sci Rep. 2017 Nov 6;7(1):14509. doi: 10.1038/s41598-017-15240-9.
Fei Liu 1,  Xiaoxin Tan 2,  Xu Han 3,  Xiang Li 2,  Nan Li 2,  Weijun Kang 4
Affiliations

Affiliations

  • 1 School of Basic Medical, Hebei University of Chinese Medicine, Shijiazhuang, Hebei Province, China.
  • 2 School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, China.
  • 3 Institute of Viral Disease, Hebei Center for Disease Control and Prevention, Shijiazhuang, Hebei Province, China.
  • 4 School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, China. [email protected].
PMID: 29109516 DOI: 10.1038/s41598-017-15240-9
Abstract

The cytotoxicities of three aconitum alkaloids- aconitine, hypaconitine and mesaconitine, and their abilities to bind DNA have been explored. Rat myocardial cells H9c2 were treated with aconitum Alkaloids and assessed the cytotoxicities by using MTT assay and flow cytometry. Apoptosis was evidenced by the results of the annexin V/propidium iodide (PI) assay. Aconitine was found to be the most toxic in rat myocardial cells H9c2 in three aconitum Alkaloids. At the same time, DNA adducts were isolated and then analyzed by UV-Vis spectroscopy after exposure to Alkaloids, which indicated that three Alkaloids could bind to DNA in rat myocardial cells H9c2. Furthermore, their binding modes were investigated by UV-Visible, fluorescence, DNA melting studies and ionic strength effect. Results indicated that the interaction between three Alkaloids and DNA were intercalation coupled with electrostatic effect. The estimated binding constants were between 4.83 × 105 M-1 to 9.85 × 105 M-1 for three Alkaloids at 298 K.

Figures
Products