2. Academic Validation
  3. Effect of BCLAF1 on HDAC inhibitor LMK-235-mediated apoptosis of diffuse large B cell lymphoma cells and its mechanism

Effect of BCLAF1 on HDAC inhibitor LMK-235-mediated apoptosis of diffuse large B cell lymphoma cells and its mechanism

  • Cancer Biol Ther. 2018;19(9):825-834. doi: 10.1080/15384047.2018.1472188.
Xinyao Li 1 2 3,  Zhengchang He 1 2 3,  Bingqing Cheng 4 2 3,  Qin Fang 4 5,  Dan Ma 1 2 3,  Tingting Lu 1 2 3,  Danna Wei 1 2 3,  Xingyi Kuang 1 2 3,  Sishi Tang 1 2 3,  Jie Xiong 1 2 3,  Jishi Wang 1 2 3
Affiliations

Affiliations

  • 1 a Guizhou Medical University , Guiyang , Guizhou , China.
  • 2 d Guizhou Province Laboratory of Haematopoietic Stem Cell Transplantation Center , Guiyang , Guizhou , China.
  • 3 e Department of Hematology , Affiliated Hospital of Guizhou Medical University , Guiyang , Guizhou , China.
  • 4 b Department of Pharmacy , Guizhou Medical University , Guiyang , Guizhou , China.
  • 5 c Department of Pharmacy , Affiliated BaiYun Hospital of Guizhou Medical University , Guiyang , Guizhou , China.
PMID: 29969367 DOI: 10.1080/15384047.2018.1472188
Abstract

Diffuse large B-cell lymphoma (DLBCL) is the most common type of adult lymphoma. It is a group of malignant tumors with a large number of clinical manifestations and prognoses. Therefore, it is necessary to explore its unknown potential therapeutic targets. Histone deacetylase inhibitor (HDACi) is a novel drug for the treatment of DLBCL, however pan-HDACis cannot be ignored because of their clinical efficacy. By contrast, specific HDACi is well-tolerated, and LMK-235 is a novel HDACi that is a specific inhibitor of HDAC4 and HDAC5. In this study, we investigated the up-regulation of BCLAF1 through NF-κB signaling pathways in LMK-235, mediating the Apoptosis of two diffuse large B-cell lymphoma cell lines, OCI-LY10 and OCI-LY3. Further studies showed that BCLAF1 expression was increased in DLBCL cells after treatment with the NF-κB Inhibitor Bay11-7082. The combination of Bay11-7082 and siRNA si-HDAC4 significantly increased BCLAF1 expression and further increased Apoptosis. These results indicate that BCLAF1 plays an important role in LMK-235-mediated Apoptosis and may be a potential target for the treatment of diffuse large B-cell lymphoma.

Keywords

BCLAF1; HDAC4; LMK-235; NF-kB; apoptosis; diffuse large B cell lymphoma.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-18998
    99.61%, HDAC4/5 Inhibitor