2. Academic Validation
  3. Stabilization of microtubules by cevipabulin

Stabilization of microtubules by cevipabulin

  • Biochem Biophys Res Commun. 2019 Aug 27;516(3):760-764. doi: 10.1016/j.bbrc.2019.06.095.
Syeda Rubaiya Nasrin 1,  Arif Md Rashedul Kabir 2,  Akihiko Konagaya 3,  Tsukasa Ishihara 4,  Kazuki Sada 5,  Akira Kakugo 6
Affiliations

Affiliations

  • 1 Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan.
  • 2 Faculty of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan.
  • 3 Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology, Yokohama, 226-8502, Japan.
  • 4 Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8566, Japan.
  • 5 Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan; Faculty of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan.
  • 6 Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan; Faculty of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan. Electronic address: [email protected].
PMID: 31253401 DOI: 10.1016/j.bbrc.2019.06.095
Abstract

We report the utility of cevipabulin as a stabilizing agent for microtubules. Cevipabulin-stabilized microtubules were more flexible compared to the microtubules stabilized by paclitaxel, the most commonly used microtubule stabilizing agent. Similar to the paclitaxel-stabilized microtubules, cevipabulin-stabilized microtubules were driven by kinesins in an in vitro gliding assay. The velocity of cevipabulin-stabilized microtubules was significantly higher than that of paclitaxel-stabilized microtubules. These findings will enrich the variety of microtubules with difference in mechanical and dynamic properties and widen their applications in nanotechnology.

Keywords

Cevipabulin; Kinesin; Microtubule; Motility assay; Persistence length.

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