2. Academic Validation
  3. C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction

C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction

  • Cell Death Dis. 2019 Jul 11;10(7):530. doi: 10.1038/s41419-019-1760-5.
Zhengbin Zhang 1,  Liwen Zhu 2 3,  Pan Feng 2,  Yanzhen Tan 2,  Bing Zhang 2,  Erhe Gao 4,  Xiaowu Wang 2,  Chongxi Fan 5,  Xiaoming Wang 1,  Wei Yi 6,  Yang Sun 7
Affiliations

Affiliations

  • 1 Department of Geriatric, Xijing Hospital, the Fourth Military Medical University, 710032, Xi'an, China.
  • 2 Department of Cardiovascular Surgery, Xijing Hospital, the Fourth Military Medical University, 710032, Xi'an, China.
  • 3 Department of Cardiology, The First Affiliated Hospital of Xi'an Medical University, 710077, Xi'an, China.
  • 4 Center for Translational Medicine, Lewis Katz School of Medicine at Temple University, 19140, Philadelphia, PA, USA.
  • 5 Department of Biomedical Engineering, the Fourth Military Medical University, 710032, Xían, China.
  • 6 Department of Cardiovascular Surgery, Xijing Hospital, the Fourth Military Medical University, 710032, Xi'an, China. [email protected].
  • 7 Department of Geriatric, Xijing Hospital, the Fourth Military Medical University, 710032, Xi'an, China. [email protected].
PMID: 31296837 DOI: 10.1038/s41419-019-1760-5
Abstract

Mesenchymal stromal cells (MSCs) transplantation offers an attractive alternative in myocardial infarctive therapy. However, poor cell engraftment and survival limit their restorative capacity. C1q/tumor necrosis factor-related protein-3 (CTRP3) inhibits reverse remodeling after myocardial infarction (MI) and was found to be secreted by MSCs in our preliminary experiments. We examined whether the overexpression of CTRP3 improved the survival of transplanted MSCs and augmented their efficacy on MI and whether silencing CTRP3 attenuated these effects. For gain-of-function analysis, MSCs overexpressing CTRP3 (LvC3-MSCs), control virus-transfected MSCs (LvNull-MSCs), MSCs alone, or phosphate-buffered saline (PBS) were injected into the peripheral areas of the infarction immediately after coronary artery ligation. For loss-of-function analysis, mice subjected to MI were randomized into groups and administered CTRP3-knockdown MSCs (LvshC3-MSCs), Lvshctrl-MSCs, MSCs, or PBS. Survival rates, cardiac function, and myocardial remodeling in mice were evaluated after 4 weeks. Injection of MSCs or LvNull-MSCs improved the left ventricular ejection fraction, inhibited cardiac fibrosis, and regulated cellular profiles of the infarction border zone 4 weeks after MI compared with those in the PBS group. Furthermore, overexpression of hCTRP3 promoted the efficacy of MSCs in the treatment of MI. However, knocking down CTRP3 impaired that. Coculture experiments confirmed that hCTRP3-enriched conditioned medium (CM) promoted MSCs migration and protected against H2O2-induced cell damage. Conversely, CM from C3-/- MSCs (CTRP3 knock out) significantly reduced the migration and antioxidative effects of MSCs. CTRP3 protein alone promoted MSCs proliferation and migration by upregulating matrix metalloproteinase 9 (MMP9) and protecting against oxidation by increasing superoxide dismutase 2 (SOD2) and metallothionein 1/2 (MT1/2) expression; and these effects were blocked by pretreatment with the extracellular signal-regulated kinase (ERK1/2) inhibitor U0126. Overexpression of CTRP3 significantly improved the MSCs-based efficacy on MI by increasing cell survival and retention via a mechanism involving ERK1/2-MMP9 and ERK1/2-SOD2/MT1/2 signaling.

Figures
Products