2. Academic Validation
  3. The effect of Guanxin Shutong capsule on alleviating the myocardial fibrosis in heart failure rats

The effect of Guanxin Shutong capsule on alleviating the myocardial fibrosis in heart failure rats

  • J Ethnopharmacol. 2021 Jul 15;275:114169. doi: 10.1016/j.jep.2021.114169.
Yuming Pan 1,  Chongyu Shao 2,  Ling Zhang 3,  Yu He 4,  Jintao Yang 5,  Wei Fu 6,  Jiehong Yang 7,  Haitong Wan 8
Affiliations

Affiliations

  • 1 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
  • 2 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
  • 3 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
  • 4 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
  • 5 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
  • 6 Department of Cardiac-Cerebral Diseases, Yinchuan Cardiac-Cerebral Treatment Internet Hospital, Yinchuan, China. Electronic address: [email protected].
  • 7 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
  • 8 Zhejiang Chinese Medical University, Hangzhou, 310053, China. Electronic address: [email protected].
PMID: 33932513 DOI: 10.1016/j.jep.2021.114169
Abstract

Ethnopharmacological relevance: Guanxin Shutong (GXST) capsule is a renowned traditional Chinese medicine widely used for the treatment of cardiovascular diseases in the clinic. However, no pharmacological experimental studies of GXST has been reported on the treatment of pressure overload-induced heart failure. This study aimed to investigate the effects of GXST capsule on ameliorating myocardial fibrosis conditions in pressure overload-induced heart failure rats.

Material and methods: Rats were randomly divided into 6 groups: Normal group, Model group, GXST-treated group at a dose of 0.5 g/kg, 1 g/kg, 2 g/kg, respectively, and digoxin positive control group at a dose of 1 mg/kg. After 4 weeks of administration, cardiac function was evaluated by echocardiography. Cardiac injury and fibrotic conditions were evaluated by H&E staining, Masson staining, and Sirius Red staining. Myocardial fibrosis was evaluated by immunohistochemistry staining and Western blot.

Results: GXST significantly inhibited cardiac fibrosis, reduced the excessive deposition of collagen, and finally improved cardiac function. GXST reversed ventricular remodeling might be through the TGF-β/SMAD3 pathway.

Conclusion: GXST capsule demonstrated a strong anti-fibrosis effect in heart failure rats by inhibiting the TGF-β/SMAD3 signaling pathway.

Keywords

Fibrosis; Guanxin shutong capsule; Heart failure; Transverse aortic constriction.

Figures
Products