2. Academic Validation
  3. Profiling alkaloids in Aconitum pendulum N. Busch collected from different elevations of Qinghai province using widely targeted metabolomics

Profiling alkaloids in Aconitum pendulum N. Busch collected from different elevations of Qinghai province using widely targeted metabolomics

  • Phytochemistry. 2022 Mar:195:113047. doi: 10.1016/j.phytochem.2021.113047.
Jun-Jie Wang 1,  Hua-Yong Lou 2,  Ying Liu 3,  Hong-Ping Han 4,  Feng-Wei Ma 2,  Wei-Dong Pan 5,  Zhi Chen 6
Affiliations

Affiliations

  • 1 Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Normal University, Xining, 810008, PR China; Bijie Medical College, Bijie, 551700, PR China.
  • 2 State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, PR China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, 550014, PR China.
  • 3 Bijie Medical College, Bijie, 551700, PR China.
  • 4 Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Normal University, Xining, 810008, PR China.
  • 5 State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, PR China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang, 550014, PR China. Electronic address: [email protected].
  • 6 Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Normal University, Xining, 810008, PR China. Electronic address: [email protected].
PMID: 34896812 DOI: 10.1016/j.phytochem.2021.113047
Abstract

Aconitum pendulum N. Busch (Ranunculaceae) is rich in Alkaloids with anti-inflammatory and analgesic activities. Many studies have focused on the identification or quantification of alkaloid components using low-throughput tests. However, the metabolic differences of Plants from environmentally distinct regions remain unclear. The present study profiled alkaloid chemical compounds in the rhizomes of A. pendulum from different regions. A total of 80 chemical compounds were identified using a widely targeted ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach. Principal component, hierarchical clustering, and orthogonal partial least squares-discriminant analyses of the chemical compounds indicated that the Plants from 6 regions clearly separated into distinct groups. A total of 19 compounds contributed the most to the metabolite differences between collection areas and were identified as potential metabolic markers. The anti-inflammatory activities of the A. pendulum extracts were also evaluated and the potential environmental effects on the regulation of metabolite composition and bioactivity were explored. These results improve our understanding of the variation in chemical composition of Plants from different regions and will serve as a reference for evaluating the medicinal value of A. pendulum in different environments.

Keywords

Aconitum pendulum N. Busch; Anti-inflammatory activity; Differential metabolites; Metabolomic profiling; Ranunculaceae; Regional environment.

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