2. Academic Validation
  3. A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1

A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1

  • Commun Biol. 2022 Apr 4;5(1):309. doi: 10.1038/s42003-022-03263-6.
Yifeng Yang  # 1,  Wenwen Lu  # 2,  Mei Ning  # 3 4,  Xianhao Zhou  # 5,  Xinyao Wan 5,  Qianglong Mi 5,  Xiaoyan Yang 2,  Di Zhang 3 4,  Yuanyuan Zhang 5,  Biao Jiang 5,  Lin He 3 4,  Jia Liu 6 7 8,  Yan Zou 9
Affiliations

Affiliations

  • 1 Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, 201210, Shanghai, China. [email protected].
  • 2 School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China.
  • 3 Key laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, 1954 HuaShan Road, Shanghai, 200030, China.
  • 4 Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
  • 5 Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, 201210, Shanghai, China.
  • 6 Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, 201210, Shanghai, China. [email protected].
  • 7 School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China. [email protected].
  • 8 Shanghai Clinical Research and Trial Center, Shanghai, China. [email protected].
  • 9 School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China. [email protected].
  • # Contributed equally.
PMID: 35379867 DOI: 10.1038/s42003-022-03263-6
Abstract

The aberrant expression or genomic mutations of MicroRNA are associated with several human diseases. This study analyzes the relationship between genetic variations of miRNA and schizophrenia or bipolar disorder. We performed case-control studies for ten SNPs in a total sample of 1584 subjects. All these ten SNPs were on or near mature MicroRNAs. We identified the association between bipolar disorder and the T/C polymorphism at rs895819. To illustrate the function of miR-27a, we constructed several miR-27a knockout (KO) cell lines, determined candidates of miR-27a, and then verified NCAM1 as a target gene of miR-27a. Further studies revealed that the T/C polymorphism on miR-27a led to the differential expression of mature and precursor miR-27a without affecting the expression of primary miR-27a. Furthermore, the C mutation on pre-miR-27a suppresses cell migration and dopamine expression levels. Our study highlights the importance of miR-27a and its polymorphism at rs895819 in bipolar disorder.

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