2. Academic Validation
  3. 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer

4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer

  • Cell Death Dis. 2022 Dec 27;13(12):1075. doi: 10.1038/s41419-022-05466-5.
Yanan Wang 1 2 3,  Jiapeng Lei 4,  Song Zhang 5,  Xiaomei Wang 6,  Jiangbo Jin 7,  Yufeng Liu 8,  Mingxi Gan 8,  Yi Yuan 9,  Longhua Sun 10,  Xiaolei Li 1,  Tianyu Han 11 12 13,  Jian-Bin Wang 14 15
Affiliations

Affiliations

  • 1 Jiangxi Institute of Respiratory Disease, The First Affiliated Hospital of Nanchang University, Nanchang City, 330006, Jiangxi, China.
  • 2 Jiangxi Hospital of China-Japan Friendship Hospital, Nanchang City, 330052, Jiangxi, China.
  • 3 Jiangxi Clinical Research Center for Respiratory Diseases, Nanchang City, 330006, Jiangxi, China.
  • 4 School of Basic Medical Sciences, Nanchang Medical College, Nanchang City, 330006, Jiangxi, China.
  • 5 Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, 310009, Zhejiang, China.
  • 6 Department of Pharmacy, Liaocheng People's Hospital, Liaocheng City, 252000, Shandong, China.
  • 7 Department of Thoracic Surgery, The First Affifiliated Hospital of Nanchang University, Nanchang City, 330006, Jiangxi, China.
  • 8 School of Basic Medical Sciences, Nanchang University, Nanchang City, 330031, Jiangxi, China.
  • 9 Huankui Academy, Nanchang University, Nanchang City, 330031, Jiangxi, China.
  • 10 Departments of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Nanchang University, Nanchang City, 330006, Jiangxi, China.
  • 11 Jiangxi Institute of Respiratory Disease, The First Affiliated Hospital of Nanchang University, Nanchang City, 330006, Jiangxi, China. [email protected].
  • 12 Jiangxi Hospital of China-Japan Friendship Hospital, Nanchang City, 330052, Jiangxi, China. [email protected].
  • 13 Jiangxi Clinical Research Center for Respiratory Diseases, Nanchang City, 330006, Jiangxi, China. [email protected].
  • 14 Department of Thoracic Surgery, The First Affifiliated Hospital of Nanchang University, Nanchang City, 330006, Jiangxi, China. [email protected].
  • 15 School of Basic Medical Sciences, Nanchang University, Nanchang City, 330031, Jiangxi, China. [email protected].
PMID: 36575176 DOI: 10.1038/s41419-022-05466-5
Abstract

Nutrient-limiting conditions are common during Cancer development. The coordination of cellular glucose levels and cell survival is a fundamental question in Cell Biology and has not been completely understood. 4EBP1 is known as a translational repressor to regulate cell proliferation and survival by controlling translation initiation, however, whether 4EBP1 could participate in tumor survival by other mechanism except for translational repression function, especially under glucose starvation conditions remains unknown. Here, we found that protein levels of 4EBP1 was up-regulated in the central region of the tumor which always suffered nutrient deprivation compared with the peripheral region. We further discovered that 4EBP1 was dephosphorylated by PTPMT1 under glucose starvation conditions, which prevented 4EBP1 from being targeted for ubiquitin-mediated proteasomal degradation by HERC5. After that, 4EBP1 translocated to cytoplasm and interacted with STAT3 by competing with JAK and ERK, leading to the inactivation of STAT3 in the cytoplasm, resulting in Apoptosis under glucose withdrawal conditions. Moreover, 4EBP1 knockdown increased the tumor volume and weight in xenograft models by inhibiting Apoptosis in the central region of tumor. These findings highlight a novel mechanism for 4EBP1 as a new cellular glucose sensor in regulating Cancer cell death under glucose deprivation conditions, which was different from its classical function as a translational repressor.

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