2. Academic Validation
  3. An undefined cystatin CsCPI1 from tea plant Camellia sinensis harbors antithrombotic activity

An undefined cystatin CsCPI1 from tea plant Camellia sinensis harbors antithrombotic activity

  • Biomed Pharmacother. 2023 Jan 25;159:114285. doi: 10.1016/j.biopha.2023.114285.
Mingqian Fang 1,  Jong-Ho Cha 2,  Hao-Ching Wang 3,  Peng Ye 4,  Bi Chen 4,  Mengrou Chen 5,  Wen-Hao Yang 6,  Xiuwen Yan 7
Affiliations

Affiliations

  • 1 Affiliated Cancer Institute & Hospital and Key Laboratory for Cell Homeostasis and Cancer Research of Guangdong Higher Education Institutes, Guangzhou Medical University, Guangzhou 910095, Guangdong, China; Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Key Laboratory of Bioactive Peptides of Yunnan Province, Institute of Zoology, Kunming 650107, Yunnan, China.
  • 2 Department of Biomedical Science and Engineering, Graduate School, Inha University, Incheon 22212, the Republic of Korea; Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 22212, the Republic of Korea.
  • 3 Graduate Institute of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.
  • 4 Affiliated Cancer Institute & Hospital and Key Laboratory for Cell Homeostasis and Cancer Research of Guangdong Higher Education Institutes, Guangzhou Medical University, Guangzhou 910095, Guangdong, China.
  • 5 Product Development Department, Nanjing Legend Biotech Co., Ltd., Nanjing 211100, Jiangsu, China.
  • 6 Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan. Electronic address: [email protected].
  • 7 Affiliated Cancer Institute & Hospital and Key Laboratory for Cell Homeostasis and Cancer Research of Guangdong Higher Education Institutes, Guangzhou Medical University, Guangzhou 910095, Guangdong, China. Electronic address: [email protected].
PMID: 36706630 DOI: 10.1016/j.biopha.2023.114285
Abstract

Tea consumption has been linked to a decreased risk of Cardiovascular Disease (CVD) mortality, which imposes a heavy burden on the healthcare system; however, which components in tea cause this beneficial effect is not fully understood. Here we uncovered a cystatin (namely CsCPI1), which is a cysteine proteinase inhibitor (CPI) of the tea plant (Camellia sinensis) that promotes antithrombotic activity. Since thrombosis is a common pathogenesis of fatal CVDs, we investigated the effects of CsCPI1, which showed good therapeutic effects in mouse models of thrombotic disease and ischemic stroke. CsCPI1 significantly increases endothelial cell production of nitric oxide (NO) and inhibits platelet aggregation. Notably, CsCPI1 exhibited no cytotoxicity or resistance to pH and temperature changes, which indicates that CsCPI1 might be a potent antithrombotic agent that contributes to the therapeutic effects of tea consumption against CVD. Specifically, the antithrombotic effects of CsCPI1 are distinct from the classical function of plant cystatins against herbivorous insects. Therefore, our study proposes a new potential role of cystatins in CVD prevention and treatment, which requires further study.

Keywords

Antithrombotic therapy; Camellia sinensis; Cystatin; Platelet aggregation; Tea consumption.

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