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  3. Fangchinoline Exerts Anticancer Effects on Colorectal Cancer Cells by Evoking Cell Apoptosis via Endoplasmic Reticulum Stress

Fangchinoline Exerts Anticancer Effects on Colorectal Cancer Cells by Evoking Cell Apoptosis via Endoplasmic Reticulum Stress

  • Bull Exp Biol Med. 2023 Apr 13;1-8. doi: 10.1007/s10517-023-05761-x.
Xiuling Jiang 1,  Yougen Hu 2,  Jianming Peng 1,  Xue Luo 1,  Landi Su 1,  Yuanjie Tang 3
Affiliations

Affiliations

  • 1 Medical School of Yangzhou Polytechnic College, Yangzhou, China.
  • 2 Department of Surgery of Jiangsu, Provincial Corps Hospital, Yangzhou, China.
  • 3 Department of Surgery of Jiangsu, Provincial Corps Hospital, Yangzhou, China. [email protected].
PMID: 37052856 DOI: 10.1007/s10517-023-05761-x
Abstract

We studied the anti-tumor effect of fangchinoline (FAN) against human colorectal Cancer cell lines CCL-244 and SW480 and analyzed the mechanism of FAN action. The cell viability and Apoptosis were assessed by MTT test and Annexin V-PI staining; Caspase-3 activity was measured by Western blotting. The expression of endoplasmic reticulum stress-related proteins was assessed by Real-Time PCR, Western blotting, and gene transfection. It was found that FAN inhibited cell growth and induced Apoptosis in human colorectal Cancer cell lines CCL-244 and SW480 in a dose-dependent manner. The Caspase-3 inhibitor Ac-DEVD-CHO could reverse the inhibitory effect of FAN. Moreover, FAN significantly increased the expression of endoplasmic reticulum stress-related proteins p-PERK, p-eIF2α, ATF4, and CHOP in CCL-244 and SW480 cells. In addition, endoplasmic reticulum stress inhibitor 4-phenylbutyric acid or CHOP knockdown could prevent FAN-induced Apoptosis. Thus, FAN induced Apoptosis of human colorectal Cancer through activation of endoplasmic reticulum stress.

Keywords

CHOP gene; apoptosis; colorectal cancer; endoplasmic reticulum stress; fangchinoline.

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