2. Academic Validation
  3. Resveratrol can induce differentiating phenotypes in canine oral mucosal melanoma cells

Resveratrol can induce differentiating phenotypes in canine oral mucosal melanoma cells

  • J Vet Med Sci. 2023 May 23. doi: 10.1292/jvms.22-0446.
Nana Fukuoka 1,  Tatsuya Ishida 2,  Kyota Ishii 1,  Ayami Sato 2 3,  Maria Lucia Zaidan Dagli 3,  Nantiga Virgona 2,  Tomohiro Yano 1 2
Affiliations

Affiliations

  • 1 Laboratory of Molecular Bromacology, Graduate School of Food and Nutritional Sciences, Toyo University.
  • 2 Institute of Life Innovation Studies, Toyo University.
  • 3 School of Veterinary Medicine and Animal Science, University of São Paulo.
PMID: 37225449 DOI: 10.1292/jvms.22-0446
Abstract

This report described the differentiation induction of canine oral mucosal melanoma (OMM) cells by resveratrol. Exposure of canine OMM cells to resveratrol (maximum dose: 50 μM and treatment period: 72 hr) induced differentiating features like melanocytes, and enhanced chemosensitivity against cisplatin, but alone had no influence on cell viability. Additionally, resveratrol significantly enhanced mRNA expression of key melanoma differentiation markers such as microphthalmia-associated transcription factor (MITF). Of several inhibitors against mitogen-activated protein kinase subtypes, only the c-Jun N-terminal kinase (JNK) inhibitor, SP600125, induced melanocyte-like morphological change and enhanced MITF mRNA expression. Furthermore, resveratrol also suppressed JNK activation in OMM cells by approximately 33%. Overall, these findings suggest that resveratrol induces differentiation in canine OMM cells, due to the inhibition of JNK signaling.

Keywords

c-Jun N-terminal kinase; canine oral mucosal melanoma cell; cell viability; microphthalmia-associated transcription factor; resveratrol.

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