2. Academic Validation
  3. Aflatoxin B1 affects porcine alveolar macrophage growth through the calcium signaling pathway mediated by the ceRNA regulatory network

Aflatoxin B1 affects porcine alveolar macrophage growth through the calcium signaling pathway mediated by the ceRNA regulatory network

  • Mol Biol Rep. 2023 Aug 12. doi: 10.1007/s11033-023-08672-2.
Yu Tian 1 2 3 4,  Pei-Yu Dong 2,  Sheng-Lin Liang 2,  Long Li 2,  Shu-Er Zhang 5,  Francesca Gioia Klinger 6,  Wei Shen 3,  You-Yu Yan 7 8,  Xi-Feng Zhang 9
Affiliations

Affiliations

  • 1 School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China.
  • 2 College of Veterinary medicine, Qingdao Agricultural University, Qingdao, 266109, China.
  • 3 College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao, 266109, China.
  • 4 State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock (R2BGL), College of Life Sciences, Inner Mongolia University, Hohhot, 010010, China.
  • 5 Animal Husbandry General Station of Shandong Province, Jinan, 250010, China.
  • 6 Saint Camillus International, University of Health Sciences, Via di Sant Alessandro 8, Rome, 00131, Italy.
  • 7 School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China. [email protected].
  • 8 Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, 13 Wuhan, 430023, China. [email protected].
  • 9 College of Veterinary medicine, Qingdao Agricultural University, Qingdao, 266109, China. [email protected].
PMID: 37572211 DOI: 10.1007/s11033-023-08672-2
Abstract

Background: Aflatoxin B1 (AFB1), one of the most prevalent contaminants in human and animal food, impairs the immune system, but information on the mechanisms of AFB1-mediated macrophage toxicity is still lacking.

Methods and results: In this study, for the first time, we employed whole transcriptome sequencing technology to explore the molecular mechanism by which AFB1 affects the growth of porcine alveolar macrophages (PAM). We found that AFB1 exposure reduced the proliferative capacity of PAM and prevented cell cycle progression. Based on whole transcriptome analysis, RT-qPCR, ICC and RNAi, we verified the role and regulatory mechanism of the competing endogenous RNA (ceRNA) network in the process of AFB1 exposure affecting the growth of PAM.

Conclusions: We found that AFB1 induced MSTRG.43,583, MSTRG.67,490, MSTRG.84,995, and MSTRG.89,935 to competitively bind miR-219a, miR-30b-3p, and miR-30c-1-3p, eliminating the inhibition of its target genes CACNA1S, RYR3, and PRKCG. This activated the calcium signaling pathway to regulate the growth of PAM. These results provide valuable information on the mechanism of AFB1 exposure induced impairment of macrophage function in humans and Animals.

Keywords

Aflatoxin B1; Calcium signaling pathway; Macrophages; ceRNA.

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