2. Academic Validation
  3. DBP exposure induces thyroid inflammatory impairment through activating AKT/NF-κB/NLRP3 signaling

DBP exposure induces thyroid inflammatory impairment through activating AKT/NF-κB/NLRP3 signaling

  • Ecotoxicol Environ Saf. 2023 Aug 23;264:115385. doi: 10.1016/j.ecoenv.2023.115385.
Lei Li 1,  Yunhui Xia 2,  Junhan Chen 3,  Xiaodong Han 3,  Lanxiang Hao 4,  Dongmei Li 5,  Yanmei Liu 6
Affiliations

Affiliations

  • 1 Endocrinology Department, Yancheng No.1 People's Hospital, Affiliated Hospital of Medical School, Nanjing University, Yancheng, Jiangsu 224001, China.
  • 2 Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China.
  • 3 Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, China.
  • 4 Endocrinology Department, Yancheng No.1 People's Hospital, Affiliated Hospital of Medical School, Nanjing University, Yancheng, Jiangsu 224001, China. Electronic address: [email protected].
  • 5 Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, China. Electronic address: [email protected].
  • 6 Endocrinology Department, Yancheng No.1 People's Hospital, Affiliated Hospital of Medical School, Nanjing University, Yancheng, Jiangsu 224001, China. Electronic address: [email protected].
PMID: 37625334 DOI: 10.1016/j.ecoenv.2023.115385
Abstract

Previous studies exhibited reproductive and neurodevelopmental toxicity in rats exposed to Di-n-butyl phthalate (DBP). However, the effects of DBP exposure on the other endocrine organ are still unclear. This study aimed to assess the impact of DBP exposure on the thyroid of male rats and the associated mechanisms. Here, rats were respectively treated with DBP at 0 (control), 50 (low dose), 250 (medium dose), or 500 (high dose) mg/kg/day dissolved in 1 ml quantity of corn oil by intragastrical administration for two weeks. The results demonstrated that the proliferation and inflammatory response changes were significantly different compared to the control. In vivo DBP is mainly converted to mono-n-butyl phthalate (MBP), an active form producing untoward reactions of DBP exposure. Therefore, for in vitro experiments, we treated the thyroid follicular epithelial cell line (Nthy-ori 3-1) in a temporal gradient using 1 mM MBP. Further in vitro studies showed that MBP exposure upregulated tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), as well as interleukin-1β (IL-1β) by activating Akt/NF-κB/NLRP3 signaling. Meanwhile, we detected that Pellino2 (Peli2) played an essential role in promoting the activation of NLRP3 inflammasome. Briefly speaking, this study confirmed that DBP exposure caused impaired thyroid structure and thyroid inflammation in male rats, which offered new views into the harm of DBP exposure on the endocrine organ.

Keywords

Di-n-butyl phthalate (DBP); Inflammation; Mono-n-butyl phthalate (MBP); Thyroid.

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