2. Academic Validation
  3. Circadian clock1a coordinates neutrophil recruitment via nfe212a/duox-reactive oxygen species pathway in zebrafish

Circadian clock1a coordinates neutrophil recruitment via nfe212a/duox-reactive oxygen species pathway in zebrafish

  • Cell Rep. 2023 Sep 26;42(10):113179. doi: 10.1016/j.celrep.2023.113179.
An-Qi Chen 1,  Min Xue 2,  Cheng-Zeng Qiu 1,  Hao-Yi Zhang 1,  Ren Zhou 1,  Ling Zhang 1,  Zong-Jun Yin 3,  Da-Long Ren 4
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • 2 School of Life Science, Anhui Medical University, Hefei 230032, China.
  • 3 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Electronic address: [email protected].
  • 4 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Electronic address: [email protected].
PMID: 37756160 DOI: 10.1016/j.celrep.2023.113179
Abstract

Neutrophil recruitment to inflammatory sites appears to be an evolutionarily conserved strategy to fight against exogenous insults. However, the rhythmic characteristics and underlying mechanisms of neutrophil migration on a 24-h timescale are largely unknown. Using the advantage of in vivo imaging of zebrafish, this study explored how the circadian gene clock1a dynamically regulates the rhythmic recruitment of neutrophils to inflammatory challenges. We generated a clock1a mutant and found that neutrophil migration is significantly increased in caudal fin injury and lipopolysaccharide (LPS) injection. Transcriptome sequencing, chromatin immunoprecipitation (ChIP), and dual-luciferase reporting experiments suggest that the clock1a gene regulates neutrophil migration by coordinating the rhythmic expression of nfe212a and duox genes to control the Reactive Oxygen Species (ROS) level. This study ultimately provides a visual model to expand the understanding of the rhythmic mechanisms of neutrophil recruitment on a circadian timescale in a diurnal organism from the perspective of ROS.

Keywords

CP: Immunology; CP: Molecular biology; ROS; circadian clock; neutrophil; nfe212a; zebrafish.

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