2. Academic Validation
  3. IL-27 regulates NLRP3 inflammasome activation of MDSCs in experimental Sjögren's syndrome

IL-27 regulates NLRP3 inflammasome activation of MDSCs in experimental Sjögren's syndrome

  • Int Immunol. 2023 Sep 26:dxad037. doi: 10.1093/intimm/dxad037.
Xiaoyu Xia 1,  Qiqiang Long 2,  Jie Zha 3,  Tingting Jiang 4,  Junqiao Guo 4,  Bo Jiang 1,  Xiaojing Li 1,  Genhong Yao 1 2
Affiliations

Affiliations

  • 1 Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
  • 2 Department of Hematology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, China.
  • 3 Teaching and Research Section of Pathogen Biology and Immunology, Basic Medical College, Anqing Medical College, Anqing 246052, China.
  • 4 Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
PMID: 37756640 DOI: 10.1093/intimm/dxad037
Abstract

Excessive NLRP3 inflammasome activation has an important function in the pathogenesis of Sjögren's syndrome (SS). Increased and dysfunctional myeloid-derived suppressor cells (MDSCs) promoted SS. However, NLRP3 inflammasome activation of MDSCs in SS and its regulated components are unclear. Splenic MDSCs were purified by immunomagnetic beads and cultured. Western-blot was used to assess NLRP3 inflammasomes. IL-1β and IL-18 were measured using enzyme-linked immunosorbent assay. Here we showed that the NLRP3 Inflammasome was activated in NOD mice with SS-like manifestations. We found that NLRP3 inflammasome activation was augmented in MDSCs of SS mice and NLRP3 inflammasome activation was suppressed in IL-27-deficient NOD mice. Consistent with findings of SS mice in vivo, we observed that NLRP3 inflammasome activation by ATP and LPS was remarkably intensified in MDSCs with IL-27 treatment in vitro. Collectively, our data highlighted that IL-27 regulates NLRP3 inflammasome activation of MDSCs in experimental SS.

Keywords

interleukin-27; myeloid-derived suppressor cells.

Figures
Products