2. Academic Validation
  3. Low-energy LED red light inhibits the NF-κB pathway and promotes hPDLSCs proliferation and osteogenesis in a TNF-α environment in vitro

Low-energy LED red light inhibits the NF-κB pathway and promotes hPDLSCs proliferation and osteogenesis in a TNF-α environment in vitro

  • Lasers Med Sci. 2023 Oct 18;38(1):240. doi: 10.1007/s10103-023-03880-5.
Yuan Liu # 1 2 3,  Juan Yang # 4 5,  Bing Jiang 4 6,  Genzi Zheng 4 7,  Yao Wang 8 9 10
Affiliations

Affiliations

  • 1 Institute of Stomatology, Southwest Medical University, Luzhou, 646000, China.
  • 2 The Third Hospital of Mianyang, Department of Stomatology, Mianyang, 621000, China.
  • 3 Sichuan Mental Health Center, Mianyang, 621000, China.
  • 4 Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou, 646000, China.
  • 5 Chenjiaqiao Hospital of Shapingba District Chongqing, Chongqing, 400000, China.
  • 6 Dazhou Hospital of Integrated TCM & Western Medicine Hospital, Dazhou, 635000, China.
  • 7 The Third Hospital of Yibin, Department of Stomatology, Yibin, 644000, China.
  • 8 Institute of Stomatology, Southwest Medical University, Luzhou, 646000, China. [email protected].
  • 9 Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou, 646000, China. [email protected].
  • 10 The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, 646000, China. [email protected].
  • # Contributed equally.
PMID: 37851127 DOI: 10.1007/s10103-023-03880-5
Abstract

There are few studies on the effect of low-energy LED red LIGHT on periodontal tissue regeneration in an inflammatory environment. In this study, Cell Counting Kit-8 (CCK-8) assays were used to detect the effects of TNF-α at three different concentrations (0, 10 ng/ml, and 20 ng/ml) on the proliferation of human periodontal ligament stem cells (hPDLSCs), and 10 ng/ml was selected as the subsequent experimental stimulation concentration. CCK-8 assays were used to detect the effect of LED red LIGHT with energy density of 1 J/ cm2, 3 J/ cm2, and 5 J/cm2 on the proliferation of hPDLSCs. The promotion effect of energy density of 5 J/cm2 on the proliferation of hPDLSCS was the most obvious (p < 0.05). Set CON group, ODM group, ODM + 10 ng/ml TNF-α group, and ODM + 10 ng/ml TNF-α + 5 J/ cm2 LED red LIGHT group. Alkaline Phosphatase staining and activity detection, alizarin red staining and calcium nodules quantitative detection of osteoblast differentiation products, real-time fluorescence quantitative PCR detection of osteoblast gene expression (Runx2, Col-I, OPN, OCN). The results showed that ODM showed the strongest osteoblast ability, followed by ODM + 10 ng/ml TNF-α + 5 J/ cm2 LED red LIGHT group. The osteoblast ability of ODM + 10 ng/ml TNF-α was decreased, but was not found in CON group. Western blot was used to detect the expression of NF-κB pathway protein and osteoblast-related proteins (Runx2, Col-I, OPN, OCN) after addition of PDTC inhibitor. The results showed that the expression of p-IκBα was increased and the expression of IκBα was decreased (p < 0.05). The expression of osteoblast protein increased after the addition of inhibitor (p < 0.05). Therefore, in an inflammatory environment constructed by 10 ng/ml TNF-α, 5 J/cm2 LED red LIGHT can upregulate the proliferation and osteogenesis of hPDLSCs by inhibiting NF-κB signaling pathway.

Keywords

LED red light; NF-κB; Osteogenic differentiation; Proliferation; TNF-α; hPDLSCs.

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