2. Academic Validation
  3. A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response

A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response

  • J Nanobiotechnology. 2023 Nov 14;21(1):424. doi: 10.1186/s12951-023-02210-9.
Jinxing Song # 1,  Mengxiang Wang # 1,  Lei Zhou # 1,  Panpan Tian 1,  ZhuoYa Sun 1,  Junru Sun 1,  Xuannian Wang 1 2,  Guoqing Zhuang 1 3,  Dawei Jiang 1 2 3,  Yanan Wu 4 5,  Gaiping Zhang 6 7 8 9
Affiliations

Affiliations

  • 1 International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
  • 2 Longhu Laboratory of Advanced Immunology, Zhengzhou, 450046, China.
  • 3 Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
  • 4 International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. [email protected].
  • 5 Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. [email protected].
  • 6 International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. [email protected].
  • 7 Longhu Laboratory of Advanced Immunology, Zhengzhou, 450046, China. [email protected].
  • 8 Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. [email protected].
  • 9 School of Advanced Agricultural Sciences, Peking University, Beijing, 100871, China. [email protected].
  • # Contributed equally.
PMID: 37964304 DOI: 10.1186/s12951-023-02210-9
Abstract

The African swine fever (ASF) pandemics pose a significant threat to the global swine industry, and the development of safe and effective vaccines is a daunting but necessary challenge. The level and persistence of immunity are very important for the effectiveness of the vaccine. Targeting antigens to antigen presenting cells (APCs) can greatly enhance immunogenicity. In this study, we developed a self-assembled nano-ASFV vaccine candidate (NanoFVax) targeting DCs, by covalently coupling the self-assembled 24-mer ferritin with the dominant B and T cell epitopes of the highly immunogenic ASFV antigen (p72, CD2v, pB602L and p30) and fused with the Chemokine Receptor XCL1 (a DC targeting molecule) through the SpyTag/SpyCatcher protein ligase system. Compared to monomeric protein, the nanoparticle vaccines can induce a more robust T-cell response, and the high-level antibody response against ASFV can last for more than 231 days. Therefore, the NanoFVax is a novel and promising vaccine candidate for ASFV.

Keywords

African swine fever; Ferritin; NanoFVax; Nanoparticles; Vaccine candidate; XCL1.

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