2. Academic Validation
  3. The Influence of Extracellular Vesicles Secreted by Dural Cells on Osteoblasts

The Influence of Extracellular Vesicles Secreted by Dural Cells on Osteoblasts

  • Mol Biotechnol. 2023 Dec 1. doi: 10.1007/s12033-023-00974-x.
Fangning Zhao 1,  Jinglin Zhu 2,  Xinhang Dong 1,  Xiaoshuang Guo 1,  Chenzhi Lai 1,  Jingyi Zhao 1,  Xianlei Zong 1,  Guodong Song 1,  Xiaolei Jin 3
Affiliations

Affiliations

  • 1 The 4th Craniomaxillofacial Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.
  • 2 The Adipose Remodeling Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.
  • 3 The 4th Craniomaxillofacial Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China. [email protected].
PMID: 38040933 DOI: 10.1007/s12033-023-00974-x
Abstract

To explore the influence of extracellular vesicles secreted by dural cells (Dura-EVs) on osteoblasts. Our methodology involves assessing the effects of these EVs at concentrations of 50ug/ml, 100ug/ml, and 200ug/ml on osteoblasts proliferation, differentiation, migration, osteogenesis, and inhibition of Apoptosis. We also treated a cranial defect model with injections of these Dura-EVs and monitored the healing rate of cranial defects. Tissue sections were analyzed using Hematoxylin and Eosin (H & E), Masson's trichrome, and immunofluorescence (IF) staining. Our results suggest that Dura-EVs can enhance osteoblasts proliferation, migration, differentiation, and osteogenesis in a dose-dependent manner in vitro. In vivo, Dura-EVs may promote the repair of skull defects. Dura-EVs have an important influence on osteoblasts, our findings shed LIGHT on a novel aspect of the dura mater's contribution to cranial osteogenesis.

Keywords

Dural cells; Exosomes; Extracellular vesicles; Osteoblasts.

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