2. Academic Validation
  3. Optimized preparation pipeline for emergency phage therapy against Pseudomonas aeruginosa at Yale University

Optimized preparation pipeline for emergency phage therapy against Pseudomonas aeruginosa at Yale University

  • Sci Rep. 2024 Feb 1;14(1):2657. doi: 10.1038/s41598-024-52192-3.
Silvia Würstle 1 2 3 4,  Alina Lee 1 2,  Kaitlyn E Kortright 1 2,  Franziska Winzig 2 4,  William An 2,  Gail L Stanley 1 3,  Govindarajan Rajagopalan 1 3,  Zach Harris 3,  Ying Sun 3,  Buqu Hu 3,  Michael Blazanin 1 2,  Maryam Hajfathalian 5,  Paul L Bollyky 5,  Paul E Turner 1 2 3 6,  Jonathan L Koff # 7 8,  Benjamin K Chan # 9 10
Affiliations

Affiliations

  • 1 Yale Center for Phage Biology and Therapy, Yale University, 165 Prospect Street, New Haven, CT, 06520, USA.
  • 2 Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, 06520, USA.
  • 3 Department of Internal Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Yale School of Medicine, New Haven, CT, 06519, USA.
  • 4 Technical University of Munich, 81675, Munich, Germany.
  • 5 Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA, 94305, USA.
  • 6 Program in Microbiology, Yale School of Medicine, New Haven, CT, 06520, USA.
  • 7 Yale Center for Phage Biology and Therapy, Yale University, 165 Prospect Street, New Haven, CT, 06520, USA. [email protected].
  • 8 Department of Internal Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Yale School of Medicine, New Haven, CT, 06519, USA. [email protected].
  • 9 Yale Center for Phage Biology and Therapy, Yale University, 165 Prospect Street, New Haven, CT, 06520, USA. [email protected].
  • 10 Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, 06520, USA. [email protected].
  • # Contributed equally.
PMID: 38302552 DOI: 10.1038/s41598-024-52192-3
Abstract

Bacteriophage therapy is one potential strategy to treat antimicrobial resistant or persistent Bacterial infections, and the year 2021 marked the centennial of Felix d'Hérelle's first publication on the clinical applications of phages. At the Center for Phage Biology & Therapy at Yale University, a preparatory modular approach has been established to offer safe and potent phages for single-patient investigational new drug applications while recognizing the time constraints imposed by Infection(s). This study provides a practical walkthrough of the pipeline with an Autographiviridae phage targeting Pseudomonas aeruginosa (phage vB_PaeA_SB, abbreviated to ΦSB). Notably, a thorough phage characterization and the evolutionary selection pressure exerted on bacteria by phages, analogous to Antibiotics, are incorporated into the pipeline.

Figures
Products