2. Academic Validation
  3. Protective effect of Enterococcus faecium against ethanol-induced gastric injury via extracellular vesicles

Protective effect of Enterococcus faecium against ethanol-induced gastric injury via extracellular vesicles

  • Microbiol Spectr. 2024 Mar 15:e0389423. doi: 10.1128/spectrum.03894-23.
Meiying Luo # 1,  Junhang Sun # 1,  Suqian Li 1,  Limin Wei 2,  Ruiping Sun 3,  Xin Feng 1,  Huihua Zhang 1,  Ting Chen 4,  Qianyun Xi 4,  Yongliang Zhang 4,  Qien Qi 1
Affiliations

Affiliations

  • 1 School of Life Science and Engineering, Foshan University, Foshan, China.
  • 2 Sanya Institute, Hainan Academy of Agricultural Sciences (Hainan Experimental Animal Research Center), Sanya, China.
  • 3 Institute of Animal Science and Veterinary Medicine of Hainan Academy of Agricultural Sciences, Haikou, China.
  • 4 Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, China.
  • # Contributed equally.
PMID: 38488394 DOI: 10.1128/spectrum.03894-23
Abstract

Recently, Enterococcus has been shown to have gastric protective functions, and the mechanisms by which Enterococcus modulates gastric function are still being investigated. Herein, we investigated how Enterococcus faecium (Efm) and E. faecium-derived extracellular vesicles (EVs) (EfmEVs) exert protective effect against ethanol-induced gastric injury by investigating the effect of EfmEVs on gastric mucosal ulcer scoring, histological lesion, mucosal glycoprotein production, acidity, anti-oxidative function, and inflammatory responses in rat. Pretreatment with Efm showed significant reduction of ethanol-induced gastric injury, as evidenced by the lowering of ulcer index, histological lesion, gastric pH, and inflammatory responses and the enhancement of mucosal glycoprotein production and anti-oxidative function. Further functional studies on three bioactive components [inactivated Efm, EfmEVs (EVs), and EV-free supernatants] of the Bacterial culture showed that EVs are mostly responsible for the gastroprotective effect. Moreover, EV secretion is beneficial for the gastroprotective effect of Efm. Hence, EVs mediated the protective effect of Efm against ethanol-induced gastric injury by lowering inflammatory responses and enhancing anti-oxidative function and may be a potent anti-inflammatory and anti-oxidative strategy to alleviate hyperinflammatory gastrointestinal tract conditions.IMPORTANCEThis study indicated that Enterococcus faecium provided a protective effect against rat gastric injury, which involved improvement of the mucosal glycoprotein production, anti-oxidative function, and inflammatory responses. Furthermore, we confirmed that three bioactive components (inactivated Efm, extracellular vesicles, and EV-free supernatants) of E. faecium culture also contributed to the gastroprotective effect. Importantly, E. faecium-derived EVs showed an effective impact for the gastroprotective effect.

Keywords

Enterococcus faecium; anti-inflammatory; anti-oxidative; extracellular vesicles; gastric injury.

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