2. Neuronal Signaling Metabolic Enzyme/Protease
  3. Monoamine Oxidase Endogenous Metabolite
  4. Norharmane

Norharmane  (Synonyms: Norharman; β-Carboline)

Cat. No.: HY-W008566 Purity: 99.46%
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Norharmane (Norharman), a β-carboline alkaloid, is a potent and reversible monoamine oxidase inhibitor, with IC50 values of 6.5 and 4.7 μM for MAO-A and MAO-B, respectively. Norharmane causes antidepressant responses. Norharmane is also a prospective anti-cancer photosensitizer. Norharmane alters polar auxin transport (PAT) by inhibiting PIN2, PIN3 and PIN7 transport proteins, thus causing a significant inhibitory effect on the growth of Arabidopsis thaliana seedlings.

For research use only. We do not sell to patients.

Norharmane Chemical Structure

Norharmane Chemical Structure

CAS No. : 244-63-3

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Norharmane (Norharman), a β-carboline alkaloid, is a potent and reversible monoamine oxidase inhibitor, with IC50 values of 6.5 and 4.7 μM for MAO-A and MAO-B, respectively. Norharmane causes antidepressant responses. Norharmane is also a prospective anti-cancer photosensitizer. Norharmane alters polar auxin transport (PAT) by inhibiting PIN2, PIN3 and PIN7 transport proteins, thus causing a significant inhibitory effect on the growth of Arabidopsis thaliana seedlings[1][2][3][4][5][6].

IC50 & Target[1]

MAO-B

4.7 μM (IC50)

Microbial Metabolite

 

MAO-A

6.5 μM (IC50)

In Vitro

Norharmane attenuates quinolinic acid formation by interferon-gamma-stimulated monocytes THP-1 cells and attenuates L-kynurenine formation, with IC50 values of 51 μM and 43 μM, respectively[4].
? Norharmane has the potential for use as an antibiotic adjuvant to enhance the efficacy of conventional antibiotics to reduce pathogenic bacterial infections[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Norharmane Related Antibodies
In Vivo

Norharmane (0-10 mg/kg, IP, once) shows an anxiety- and antidepressant-like behavior, and decreased locomotor activity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NMRI mice (22-25 g)[3]
Dosage: 0, 2.5, 5 and 10 mg/kg
Administration: IP, once
Result: Elicited an anxiety- and antidepressant-like behavior, and decreased locomotor activity.
Molecular Weight

168.20

Formula

C11H8N2
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

N1C2=C(C=CC=C2)C2=C1C=NC=C2
Structure Classification
Initial Source

Cyanobacteria
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 11 mg/mL (65.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9453 mL 29.7265 mL 59.4530 mL
5 mM 1.1891 mL 5.9453 mL 11.8906 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.1 mg/mL (6.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.1 mg/mL (6.54 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.46%

COA (253 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.9453 mL 29.7265 mL 59.4530 mL 148.6326 mL
5 mM 1.1891 mL 5.9453 mL 11.8906 mL 29.7265 mL
10 mM 0.5945 mL 2.9727 mL 5.9453 mL 14.8633 mL
15 mM 0.3964 mL 1.9818 mL 3.9635 mL 9.9088 mL
20 mM 0.2973 mL 1.4863 mL 2.9727 mL 7.4316 mL
25 mM 0.2378 mL 1.1891 mL 2.3781 mL 5.9453 mL
30 mM 0.1982 mL 0.9909 mL 1.9818 mL 4.9544 mL
40 mM 0.1486 mL 0.7432 mL 1.4863 mL 3.7158 mL
50 mM 0.1189 mL 0.5945 mL 1.1891 mL 2.9727 mL
60 mM 0.0991 mL 0.4954 mL 0.9909 mL 2.4772 mL
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Norharmane Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Norharmane244-63-3Norharman β-CarbolineMonoamine OxidaseEndogenous MetaboliteMAOMonoamineoxidaseCoffeeAlkaloidParkinsondiseaseNeuroprotectionInhibitorinhibitorinhibit

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