ACPP/Prostatic Acid Phosphatase Protein, Mouse (HEK293, His)

The acid prostatic phosphatase (ACPP) protein, encapsulated in virus-like particles (VLPs), is an acid tyrosine phosphatase that skillfully dephosphorylates a variety of substrates. ACPP-VLP possesses lipid phosphatase activity and inactivates lysophosphatidic acid. ACPP/Prostatic Acid Phosphatase Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACPP/Prostatic Acid Phosphatase protein, expressed by HEK293 , with C-His labeled tag. The total length of ACPP/Prostatic Acid Phosphatase Protein, Mouse (HEK293, His) is 350 a.a., with molecular weight of 44-55 kDa.

Prostatic Acid Phosphatase (ACPP) protein serves as a versatile non-specific tyrosine phosphatase, exhibiting its catalytic activity under acidic conditions (pH 4-6) on a broad range of substrates, including alkyl, aryl, and acyl orthophosphate monoesters, as well as phosphorylated proteins. Notably, ACPP also displays lipid phosphatase activity, contributing to the inactivation of lysophosphatidic acid in seminal plasma. Beyond its tyrosine phosphatase functions, ACPP demonstrates ecto-5'-nucleotidase activity in dorsal root ganglion neurons, leading to the generation of adenosine from AMP. This extracellular adenosine, in turn, contributes to a reduction in chronic pain by activating A1R in nociceptive neurons. The multifaceted enzymatic activities of ACPP highlight its potential significance in various cellular processes and its therapeutic implications, particularly in pain management.

Measured by its ability to cleave 2mM substrate p-Nitrophenyl phosphate (pNPP). which can be measured by absorbance at 410 nm that incubate at room temperature for 5 minutes. The specific activity is 104600.068 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-6*His

Q8CE08 (K32-R381)

56318  [NCBI]

KELKFVTLVFRHGDRGPIETFPTDPITESSWPQGFGQLTQWGMEQHYELGSYIRKRYGRFLNDTYKHDQIYIRSTDVDRTLMSAMTNLAALFPPEGISIWNPRLLWQPIPVHTVSLSEDRLLYLPFRDCPRFEELKSETLESEEFLKRLHPYKSFLDTLSSLSGFDDQDLFGIWSKVYDPLFCESVHNFTLPSWATEDAMIKLKELSELSLLSLYGIHKQKEKSRLQGGVLVNEILKNMKLATQPQKYKKLVMYSAHDTTVSGLQMALDVYNGVLPPYASCHMMELYHDKGGHFVEMYYRNETQNEPYPLTLPGCTHSCPLEKFAELLDPVISQDWATECMATSSHQVLR

Approximately 44-55 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.