AGO1 Protein, Human (sf9, His)

The AGO1 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs) and inhibiting the translation of complementary mRNAs. Despite lacking endonuclease activity, AGO1 does not cleave target mRNA. AGO1 Protein, Human (sf9, His) is the recombinant human-derived AGO1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of AGO1 Protein, Human (sf9, His) is 857 a.a., with molecular weight of ~99.5 kDa.

AGO1, a pivotal component in RNA-mediated gene silencing (RNAi), functions by binding to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), thereby orchestrating the repression of mRNA translation through complementary base pairing. Notably, AGO1 lacks endonuclease activity, diverging from its counterparts, and does not participate in the cleavage of target mRNAs. Beyond post-transcriptional gene silencing, AGO1 is instrumental in transcriptional gene silencing (TGS), acting on promoter regions that exhibit complementarity to the bound short antigene RNAs (agRNAs). Its extensive network of molecular interactions involves key players such as DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1, YBX1, LIMD1, WTIP, AJUBA, APOBEC3F, APOBEC3G, and APOBEC3H. Moreover, AGO1's association with polysomes and messenger ribonucleoproteins (mNRPs) emphasizes its integral role in the dynamic regulation of gene expression at various stages.

Human

Sf9 insect cells

N-His

Q9UL18 (M1-A857)

26523  [NCBI]

Approximately 99.5 kDa

Greater than 85% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Gly, 0.5 PMSF, 0.5 mM EDTA, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.